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蚂蚁淘在线 / 品牌中心 / Athens Research / Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-CHOK-1-05

Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-CHOK-1-05

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货号：EZT-CHOK-1-05
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品牌：Athens Research
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Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

CHO Cell Avalanche™ Transfection Reagent

CHO Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on CHO Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for CHO Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of CHO Cell Avalanche™ Transfection Reagent for transfection on CHO Cells.

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-CHOK-1-05CHO Cell Transfection Reagent0.5ml£399.00QuantityAdd to Order
EZT-CHOK-1-15CHO Cell Transfection Reagent1.5ml£699.00QuantityAdd to Order

CHO Cell Avalanche™ Transfection Reagent

CHO Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on CHO Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for CHO Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of CHO Cell Avalanche™ Transfection Reagent for transfection on CHO Cells.

EZ Biosystems

Cell Line Information:

Designations: CHO Organism: Cricetulus griseus (hamster, Chinese) Tissue: ovary Gender: female Morphology: epithelial-like Growth properties: adherent VirusSuscept: vesicular stomatitis (Indiana); Getah virus VirusResist: poliovirus 2; modoc virus; Button Willow virus Reverse Transcriptase: negative Depositors: T.T. Puck Chinese hamster ovary (CHO) cells are a cell line derived from the ovary of the Chinese hamster. The CHO line was established in 1957. The Chinese hamster has a low chromosome number (2n=22) making it an excellent model for radiation cytogenetics and tissue culture. CHO are the most widely used mammalian cells for transfection, expression, and large-scale recombinant protein production. The cell line is highly sensitive to toxic material. The CHO clonal cytotoxicity test system has been validated through analysis of organic compounds, environmental samples, and metal salts.

The Transfection Reagent:

CHO Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on CHO Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for CHO Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of CHO Cell Avalanche™ Transfection Reagent for transfection on CHO Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in CHO Cells.
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on CHO-K1 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for CHO-K1 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this CHO Cell Avalanche Transfection Reagent.
FIG. 2. CHO-K1 cells were transfected with GFP vector (pEGFP-N3) by using CHO Cells Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
CHO Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

SK-OV-3 Cell Vero Cell 293 Cell 293T/17 Cell Saos-2 Cell SK-N-SH Cell B16-F10 Cell 4T1 Cell A549 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.