Athens Research/Cambio - Excellence in Molecular Biology/each/MP-KT-1009-01

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STEM Learning

STEM Learning: STEM Kits

A range of easy to use kits for PCR, gel electrophoresis and other molecular biology techniques especially designed for budding school students, in schools, 6th form colleges and other educational institutes.

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  • Description

miniPCR™ Forensics Lab-Analysis of the D1S80 VNTR

n this miniPCR Learning Lab™, students will amplify their own DNA and compare it to a sample obtained from a hypothetical crime scene in order to try to rule themselves out as a suspect. Students will use essential molecular biology techniques of PCR (polymerase chain reaction), gel electrophoresis, and VNTR (variable number tandem repeat) analysis. This lab illustrates real-world applications of molecular biology in personal identification and forensics and in the study of inheritance, human genetics, DNA polymorphisms, and genetic diversity.- A REALISTIC CRIME SCENE INVESTIGATION.

MiniPCR - Amplyus

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MP-KT-1009-01miniPCR™ Forensics Lab: Analysis of the D1S80 VNTReach£76.00QuantityAdd to Order

miniPCR™ Forensics Lab-Analysis of the D1S80 VNTR

n this miniPCR Learning Lab™, students will amplify their own DNA and compare it to a sample obtained from a hypothetical crime scene in order to try to rule themselves out as a suspect. Students will use essential molecular biology techniques of PCR (polymerase chain reaction), gel electrophoresis, and VNTR (variable number tandem repeat) analysis. This lab illustrates real-world applications of molecular biology in personal identification and forensics and in the study of inheritance, human genetics, DNA polymorphisms, and genetic diversity.- A REALISTIC CRIME SCENE INVESTIGATION.

MiniPCR - Amplyus

In this miniPCR Learning Lab, students will amplify their own DNA and compare it to a sample obtained from a hypothetical crime scene in order to try to rule themselves out as a suspect. Students will use essential molecular biology techniques of PCR (polymerase chain reaction), gel electrophoresis, and VNTR (variable number tandem repeat) analysis. This lab illustrates real-world applications of molecular biology in personal identification and forensics and in the study of inheritance, human genetics, DNA polymorphisms, and genetic diversity.

Description

A REALISTIC CRIME SCENE INVESTIGATION.

In this miniPCR Learning Lab, students will amplify their own DNA and compare it to a sample obtained from a hypothetical crime scene in order to try to rule themselves out as a suspect. Students will use essential molecular biology techniques of PCR (polymerase chain reaction), gel electrophoresis, and VNTR (variable number tandem repeat) analysis. This lab illustrates real-world applications of molecular biology in personal identification and forensics and in the study of inheritance, human genetics, DNA polymorphisms, and genetic diversity.

Variable number tandem repeats (VNTRs) are regions in a genome that contain short stretches of DNA (6 to about 100 base pairs) repeated a number of times. The number of repeats in a particular VNTR can vary from individual to individual, and between chromosomes within an individual. In this way, VNTRs are one source of genetic polymorphism (variation), and can be used as markers for personal identification as well as in the study of inheritance, genetic diversity, population genetics, and genetic disorders.

This lab is presented as a hypothetical crime where DNA evidence has been collected. Included in the lab is a sample of “Crime Scene DNA” that will be used by students to compare to their own DNA sample. Students whose DNA does not match the “Crime Scene DNA” can rule themselves out as suspects. It is possible that some students will match the DNA sample provided. These students cannot rule themselves out and are considered possible suspects.

This Learning Lab is aligned with National Standards. It utilizes the following molecular genetic techniques:

  • DNA extraction from cheek cells
  • PCR amplification of human genomic DNA
  • DNA Gel electrophoresis, staining, and visualization
  • VNTR (variable tandem number repeat) analysis

The miniPCR Forensics Lab kit contains reagents for 8 lab groups of 4 students each (32 students):

  • X-TractDNA extraction buffer
  • 2X Blue PCR Master Mix, Load-Ready
  • D1S80 Lab Primer Mix
  • “Crime Scene” DNA control sample
  • 100 bp DNA ladder, Load Ready

Please note:

  • These reagents require frozen storage.
  • Microtubes,PCR tubes, and electrophoresis reagents sold separately(or select the option above for Lab Companion Kitadd-on)

Free miniPCR Learning Labdownloads:

  • miniPCR Forensics Lab Instructor’s Guide
  • miniPCR Forensics Lab Student’s Guide
  • miniPCR Forensics Lab Classroom Slides
  • D1S80 Data Spreadsheet
  • Case Study: miniPCR use in real-world forensics
  • DNAdots: DNA Fingerprinting

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.