Athens Research/Cambio - Excellence in Molecular Biology/Kit/MP-KT-1001-03

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货号:MP-KT-1001-03
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STEM Learning

STEM Learning: STEM Kits

A range of easy to use kits for PCR, gel electrophoresis and other molecular biology techniques especially designed for budding school students, in schools, 6th form colleges and other educational institutes.

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miniPCR Genes in Space Food Safety Lab- Mars Colony at Risk

In this miniPCR Learning Lab™, you will help astronauts control an outbreak of pathogenic bacteria in space food bound for Mars! Learn how to use miniPCR™ (the PCR machine aboard the International Space Station), restriction analysis, and DNA gel electrophoresis to characterize bacterial strains and protect the health of astronauts.

MiniPCR - Amplyus

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MP-KT-1001-03miniPCR Genes in Space Food Safety Lab: Mars Colony at RiskKit£70.00QuantityAdd to Order

miniPCR Genes in Space Food Safety Lab- Mars Colony at Risk

In this miniPCR Learning Lab™, you will help astronauts control an outbreak of pathogenic bacteria in space food bound for Mars! Learn how to use miniPCR™ (the PCR machine aboard the International Space Station), restriction analysis, and DNA gel electrophoresis to characterize bacterial strains and protect the health of astronauts.

MiniPCR - Amplyus

miniPCR Genes in Space Food Safety Lab: Mars Colony at Risk

Description

In this 120-minute miniPCR Learning Lab, you will help the International Space Agency control an outbreak of pathogenic bacteria in space food bound for Mars! Learn how to use miniPCR (the PCR machine aboard the International Space Station), restriction analysis, and DNA gel electrophoresis to characterize bacterial strains and protect the health of astronauts.

This applied biotechnology investigationaddressesa foodborne illness outbreak throughthe same real-world methodologiesthat public health and surveillance authorities use here on Earth. You’ll learnmolecular microbiology techniques relevant to the STEMjob market, and engage in a real-world problem with hugepublic health and economic impact.

To learn more about miniPCR aboard the International Space Station, watch this video or visit www.genesinspace.org.

This miniPCR Learning Labutilizes the following molecular techniques:

  • Micropipetting
  • PCR (polymerase chain reaction) amplification of bacterial genes
  • Restriction digest, RFLP analysis
  • DNA Gel electrophoresis, staining, and visualization
  • Highlights real-world applications of molecular microbiology in public health.

The miniPCR Food Safety Lab kit contains reagents for 8 lab groups of 4 students:

  • 2X EZ PCR Master Mix, Load-Ready
  • 3X Food Safety Lab Primer Mix
  • 4 DNA samples to investigate, including controls
  • Restriction enzyme XmnI
  • 100 bp DNA ladder, Load-Ready

Please note:

  • These reagents require frozen storage.
  • Microtubes,PCR tubes, and electrophoresis reagents sold separately.
  • None of the materials provided in the Food Safety laboratory kit pose a health or food safety hazard. At no point are pathogenic bacteria used; references to “pathogenic” and “non-pathogenic” strains or DNA samples are used only to recreate a simulated Food Safety investigation scenario.

Free miniPCR Learning Labresources:

  • Space Food Safety Lab Instructor’s Guide
  • Space Food Safety Lab Student’s Guide
  • Space Food Safety Lab Classroom Slides
  • Genes in Space Food Safety Lab instructional videos

Optional: Lab Companion Kit

Add all the consumables you need for a class of 32 students: microtubes (50), PCR tubes (100), agarose (10 blueGel Tabs x 0.4 g ea.), 20X TBE electrophoresis buffer (30 ml), and GelGreen™ DNA stain, 10,000X (20 µl).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.