Athens Research/Cambio - Excellence in Molecular Biology/50µmoles/10-3017-95

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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.

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Pyrrolo-C-TOM-CE Phosphoramidite

Pyrrolo-C-TOM-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
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10-3017-02Pyrrolo-C-TOM-CE Phosphoramidite0.25g£980.00£855.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3017-90Pyrrolo-C-TOM-CE Phosphoramidite100µmoles£396.00£334.40Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order
10-3017-95Pyrrolo-C-TOM-CE Phosphoramidite50µmoles£206.00£167.20Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order

Pyrrolo-C-TOM-CE Phosphoramidite

Pyrrolo-C-TOM-CE Phosphoramidite

Glen Research

Pyrrolo-C-TOM-CE Phosphoramidite

Structure

Catalog Number: 10-3017-xx

Description: Pyrrolo-C-TOM-CE Phosphoramidite

5"-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2"-O-triisopropylsilyloxymethyl-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H72N5O9PSiM.W.: 970.23F.W.: 343.27

Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time recommended
Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures.Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This product is covered by US Patent No.: 7,144,995. Patented under European patent number 1483280.

MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

rSpacer is used to introduce an abasic site to an RNA sequence.

The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrrolo-C-TOM-CE Phosphoramidite

Pyrrolo-C-TOM-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 16.1: Minor Base and Related Novel Phosphoramidites

Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrrolo-C-TOM-CE Phosphoramidite

Pyrrolo-C-TOM-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.

ABI 392/394
Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µm
Approximate Number of Additions
10-3017-020.25grams.25grams2.5872.6743.627.2519.8214.533.63
10-3017-90100µmoles.097grams120127.55.4541
10-3017-9550µmoles.049grams.53.3321.25.91.67.17
Expedite
Cat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µm
Approximate Number of Additions
10-3017-020.25grams.25grams3.85.07N/AN/A14.71N/A
10-3017-90100µmoles.097grams1.5.07N/AN/A4.92N/A
10-3017-9550µmoles.049grams.75.07N/AN/A1.79N/A
Beckman
Cat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nm
Approximate Number of Additions
10-3017-020.25grams.25grams3.85.07N/A11.6510.94
10-3017-90100µmoles.097grams1.5.07N/A4.063.82
10-3017-9550µmoles.049grams.75.07N/A1.651.55

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.