- 转铁蛋白和apotranferrins
- Lab Reagents: Smart Buffers and Reagents
- 分子生物学用酶：分子生物学用酶
- 蛋白质纯化：阿波罗20毫升超滤
- 免疫球蛋白
- qPCR：基因分析和基因面板
- PCR : PCR Products
- 牛奶和血小板蛋白
- DNA & RNA Sequencing : Dye Terminator clean-up
- Aptamers: Catalogue Aptamers
- Peptide Synthesis : Amino Acids
- 运输和其他蛋白质
- DNA: Whole DNA
- STEM学习：STEM工具包
- 低内毒素水平蛋白质
- 提取和纯化-RNA：组织/培养
- 转染-EZ生物系统：细胞系
- 凝血因子
- 分析试剂盒
- 抗体
- Gel Electrophoresis: Gel Electrophoresis
Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
PC Amino-Modifier Phosphoramidite
PC Amino-Modifier Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-4906-02 | PC Amino-Modifier Phosphoramidite | 0.25g | £316.00£300.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-4906-90 | PC Amino-Modifier Phosphoramidite | 100µmoles | £108.00£102.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
PC Amino-Modifier Phosphoramidite
PC Amino-Modifier Phosphoramidite
Glen Research
| PC Amino-Modifier Phosphoramidite |
Catalog Number: 10-4906-xx
Description: PC Amino-Modifier Phosphoramidite
| [(6-Trifluoroacetylamidocaproamidomethyl)-1-(2-nitrophenyl)-ethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite | ||
| Formula: C26H39F3N5O6P | M.W.: 605.59 | F.W.: 371.32 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: 2 minute coupling time recommended |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Please Note: Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc.,617-975-0680, http://www.ambergen.com/. |
TERMINUS MODIFIERS
Glen Research 5"-Modifiers are designed for use in DNA synthesizers to functionalize the 5"-terminus of the target oligonucleotide. The 5"-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.
The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT , which is more stable than MMT , does not reattach itself to an amine. We now offer 5"-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.
5"-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.
Our more recent 5"-amino modifiers, protected by a novel phthalic acid diamide (PDA) protecting group, are stable solids . In contrast to the TFA protected amino modifiers, which are viscous oils, the analogous PDA protected compounds are granular powders. This important property of these compounds allows straightforward handling, storage and aliquoting and leads to a significant increase in stability.
Deprotection with methylamine in gas phase or aqueous solution or AMA leads to fast and complete removal of the PDA protecting group. However, ammonium hydroxide will not drive the equilibrium reaction to completion and only partial deprotection occurs - overnight deprotection with ammonium hydroxide will yield around 80% active amine.
We are offering three PDA Amino-Modifiers:
-
5"-Amino-Modifier C6-PDA
Hydrophobic 5"-Amino-Modifier C12-PDA
Hydrophilic 5"-Amino-Modifier-TEG-PDA
The disulfide thiol modifier may be used for introducing 3"- or 5"-thiol linkages. Dithiol Phosphoramidite (DTPA) is a disulfide-containing modifier designed to functionalize synthetic DNA or RNA with multiple thiol groups and can be incorporated at any position of the oligonucleotide. Each DTPA addition leads to two thiol groups. This modifier was designed for optimal tethering of oligonucleotides to a gold surface but it can also be used for multiple reactions with maleimides and other thiol-specific derivatives. 5"-Carboxy-Modifier C10 is a unique linker designed to be added at the terminus of an oligonucleotide synthesis. It generates an activated carboxylic acid N-hydroxysuccinimide (NHS) ester suitable for immediate conjugation on the synthesis column with molecules containing a primary amine, resulting in a stable amide linkage. PC Amino-Modifier is a photocleavable C6 amino-modifier, part of our line of photocleavable (PC) modifiers.
5"-AminoOxy-Modifier 11 is based on a tetraethylene glycol linkage for improved solubility and for reducing the potential negative impact on hybridization of the oligo. The oxime formed from the reaction of alkyloxyamines with aldehydes creates a stable covalent bond. In comparison, the imine formed by the conjugation of primary amines with aldehydes is not stable to acidic or basic conditions and requires subsequent reduction with borohydride to form stable amine conjugates. 5"-Maleimide Modifier Phosphoramidite, developed at the University of Barcelona, incorporates a maleimide cycloadduct that is stable to ammonium hydroxide at room temperature. This phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages. A retro–Diels-Alder reaction deprotects the maleimide immediately prior to conjugation.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
PC Amino-Modifier Phosphoramidite
PC Amino-Modifier Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 14.1: PC Biotin and Related Photocleavable Modifiers
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
PC Amino-Modifier Phosphoramidite
PC Amino-Modifier Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-4906-02 | 0.25grams | .25grams | 4.13 | 124.33 | 74.6 | 46.63 | 33.91 | 24.87 | 6.22 |
| 10-4906-90 | 100µmoles | .061grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-4906-02 | 0.25grams | .25grams | 6.16 | .07 | 116.8 | 73 | 53.09 | 7.3 | |
| 10-4906-90 | 100µmoles | .061grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-4906-02 | 0.25grams | .25grams | 6.16 | .07 | 118.4 | 74 | 53.82 | ||
| 10-4906-90 | 100µmoles | .061grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
