- 转铁蛋白和apotranferrins
- Lab Reagents: Smart Buffers and Reagents
- 分子生物学用酶：分子生物学用酶
- 蛋白质纯化：阿波罗20毫升超滤
- 免疫球蛋白
- qPCR：基因分析和基因面板
- PCR : PCR Products
- 牛奶和血小板蛋白
- DNA & RNA Sequencing : Dye Terminator clean-up
- Aptamers: Catalogue Aptamers
- Peptide Synthesis : Amino Acids
- 运输和其他蛋白质
- DNA: Whole DNA
- STEM学习：STEM工具包
- 低内毒素水平蛋白质
- 提取和纯化-RNA：组织/培养
- 转染-EZ生物系统：细胞系
- 凝血因子
- 分析试剂盒
- 抗体
- Gel Electrophoresis: Gel Electrophoresis
Oligo Synthesis : CEPs
Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.
dU-CE Phosphoramidite
dU-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1050-02 | dU-CE Phosphoramidite | 0.25g | £80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1050-90 | dU-CE Phosphoramidite | 100µmoles | £50.00£47.50Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
dU-CE Phosphoramidite
dU-CE Phosphoramidite
Glen Research
Catalog Number: 10-1050-xx
Description: dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H47N4O8P | M.W.: 730.8 | F.W.: 290.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
DUPLEX EFFECTS
The design of primers is frequently complicated by the degeneracy of the genetic code. Three strategies are now available to confront this problem. In the first, a mixed base addition (N) is used to form the degenerate site. This approach is best if the number of degenerate sites is small. A second option is the use of 2’-deoxyInosine or 2’-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other four bases. The third option is the use of a universal nucleoside. In this strategy, the base analog does not hybridize significantly to the other four bases and makes up some of the duplex destabilization by acting as an intercalating agent. 3-Nitropyrrole 2’-deoxynucleoside (M) is the first example of a set of universal bases. Subsequently, 5-nitroindole was determined to be an effective universal base and to be superior to 3-nitropyrrole, based on duplex melting experiments.
The modified bases designated P and K show considerable promise as degenerate bases. The pyrimidine derivative P, when introduced into oligonucleotides, base pairs with either A or G, while the purine derivative K base pairs with either C or T. A dP dK mix also can serve as a mixed base with much less degeneracy than dA dC dG dT (N).
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dU-CE Phosphoramidite
dU-CE Phosphoramidite
Glen Research
Material Safety Data Sheet
???Glen Report 23.1: DNA Methylation Revisited
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dU-CE Phosphoramidite
dU-CE Phosphoramidite
Glen Research
dU-CE Phosphoramidite
| 5"-Dimethoxytrityl-2"-deoxyUridine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C39H47N4O8P | M.W.: 730.8 | F.W.: 290.17 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites |
| Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1050 | dU | 262 | 10 | 10 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 3.42 | 100.67 | 60.4 | 37.75 | 27.45 | 20.13 | 5.03 |
| 10-1050-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 5.11 | .067 | 95.8 | 59.88 | 43.55 | 5.99 | |
| 10-1050-90 | 100µmoles | .073grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 5.11 | .067 | 97.4 | 60.88 | 44.27 | ||
| 10-1050-90 | 100µmoles | .073grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dU-CE Phosphoramidite
dU-CE Phosphoramidite
Glen Research
The design of primers is frequently complicated by the degeneracy of the genetic code. Three strategies are now available to confront this problem.
- A mixed base addition (N) is used to form the degenerate site. This approach is best if the number of degenerate sites is small.
- The use of 2"-deoxyInosine or 2"-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other 4 bases.
- The use of a universal nucleotide. In this strategy, the base analog does not hybridise significantly to the other 4 bases and makes up some of the duplex destabilisation by acting as an intercalating agent.
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1050 | dU | 262 | 10 | 10 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 3.42 | 100.67 | 60.4 | 37.75 | 27.45 | 20.13 | 5.03 |
| 10-1050-90 | 100µmoles | .073grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 5.11 | .07 | 95.8 | 59.88 | 43.55 | 5.99 | |
| 10-1050-90 | 100µmoles | .073grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1050-02 | 0.25grams | .25grams | 5.11 | .07 | 97.4 | 60.88 | 44.27 | ||
| 10-1050-90 | 100µmoles | .073grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
