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BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They not only promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signalling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP4 and BMP7 are also known to be involved in the differentiation of sympathetic neurons. With
an apparent molecular mass of 34 kDa, BMP4 is a disulfide linked homodimeric glycoprotein that is expressed majorly in the lungs but also found in the kidneys, normal and neoplastic prostate tissues, and prostate cancer cell lines.
Chapter 3 CELL CULTURE MEDIA 培养基
mECM1166 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml
mECM1168 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml
mECM1169 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 250 ml
mFBM2267 小鼠成纤维细胞培养基 Mouse Fibroblast Growth Medium /w BulletKit 500 ml
mBMM3366 小鼠骨髓细胞培养基 Mouse Bone Marrow Leukocyte Culture Medium /w BulletKit 500 ml
mBMM3367 小鼠骨髓中性粒细胞培养基 Mouse Bone Marrow Neutrophil Medium /w BulletKit 500 ml
mBMM3368 小鼠骨髓巨噬细胞培养基 Mouse Bone Marrow Macrophage Culture Medium /w BulletKit 500 ml
mKSM5566 小鼠骨髓间充质干细胞培养基 Mouse Bone Marrow Mesenchymal Stem Cell Medium /w BulletKit 500 ml
mBMM3369 小鼠骨髓干细胞培养基 Mouse Bone Marrow Progenitor Cell Medium /w BulletKit 500 ml
mKSM5567 小鼠细胞培养基 Mouse Cell Growth Medium /w BulletKit 500ml
mEPM6621 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml
mEPM6622 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml
mEPM6623 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 250 ml
mDCM7711 小鼠骨髓树突细胞培养基 Mouse Bone marrow-dendritic cells Medium /w BulletKit 500 ml
mESCM8811 小鼠胚胎干细胞培养基 Mouse Embryonic Stem Cell Medium /w BulletKit 500 ml
Chapter 4 CELL GROWTH SUPPLEMENT
1166 内皮细胞生长助生长溶液-ECGS Endothelial Cell Growth Supplement-ECGS 5 ml
1167 内皮细胞助生长溶液-V Endothelial Cell Growth Supplement-V 5 ml
2267 小鼠成纤维细胞生长因子 Mouse Fibroblast Growth Supplement 5 ml
3368 小鼠骨髓巨噬细胞助生长溶液 Mouse Bone Marrow Macrophage Culture Supplement 5ml
3369 小鼠骨髓干细胞助生长溶液 Mouse Bone Marrow Macrophage Growth Supplement 5ml
5566 小鼠骨髓间充质干细胞助生长溶液 Mouse Bone Marrow Mesenchymal Stem Cell Growth Supplement 5 ml
6621 小鼠上皮细胞助生长溶液-I Mouse Epithelial Cell Growth Supplement-ITS 5 ml
6622 小鼠上皮细胞助生长溶液-E Mouse Epithelial Cell Growth Supplement-EGF 5 ml
6911 胎牛血清-2 ml Fetal Bovine Serum (FBS) 2 ml
6912 胎牛血清-20 ml Fetal Bovine Serum (FBS) 25 ml
6913 胰酶/EDTA消化液 (T/E)-50 ml 0.05% Trypsin/EDTA Solution 50 ml
6914 胰酶/EDTA消化液 (T/E)-100 ml 0.05% Trypsin/EDTA Solution 100 ml
6915 非酶细胞裂解液 Enzyme-free Cell Dissociation solution 100 ml
6916 细胞冻存液 Cell Freezing Medium 50 ml
6917 青霉素/链霉素溶液-5 ml Penicillin/Streptomycin Solution 5 ml
6918 青霉素/链霉素溶液-25 ml Penicillin/Streptomycin Solution 25 ml
6919 抗霉菌溶液-25 ml Antibiotic/Antimycotic Solution 25 ml
6920 抗霉菌溶液-5 ml Antibiotic/Antimycotic Solution 5 ml
6921 左旋谷氨酰胺溶液 L-Glutamine-200 mM (100X), liquid 5 ml
6922 非必需氨基酸溶液 Non-Essential Amino Acids Solution 10 mM (100X), liquid 5 ml
6923 丙酮酸钠溶液 Sodium Pyruvate Solution 100 mM (100X), liquid 5 ml
6924 维生素溶液 Vitamin Solution (100X), liquid 5 ml
6925 2-巯基乙醇溶液 2-Mercaptoethanol (1,000X), liquid prepared in D-PBS, 5 ml
6926 胰岛素铁硒传递蛋白 Insulin-Transferrin-Selenium (100X) 5 ml
6927 牛脑垂体提取物 Bovine Pituitary Extract 25 ml
6928 HEPES 缓冲液 HEPES Buffer Solution (1 M) 5 ml
6929 胰酶/EDTA消化液 (T/E)-50 ml 0.25% Trypsin/EDTA Solution 50 ml