HamsterPrimaryStomachEpithelialCells
CatalogNo.HM-6039
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CatalogNo.M6621
ProductDescription
HamsterPrimaryStomachEpithelialCellsfromCellBIOLOGicsareisolatedfromstomachtissueof hamster.HamsterPrimaryStomachEpithelialCellsaregrowninaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumfor3-5days.Cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.
HamsterPrimaryStomachEpithelialCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
HamsterPrimaryStomachEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
HamsterPrimaryStomachEpithelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughHamsterPrimaryStomachEpithelialCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
Chapter 3 CELL CULTURE MEDIA 培养基
mECM1166 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml
mECM1168 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml
mECM1169 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 250 ml
mFBM2267 小鼠成纤维细胞培养基 Mouse Fibroblast Growth Medium /w BulletKit 500 ml
mBMM3366 小鼠骨髓细胞培养基 Mouse Bone Marrow Leukocyte Culture Medium /w BulletKit 500 ml
mBMM3367 小鼠骨髓中性粒细胞培养基 Mouse Bone Marrow Neutrophil Medium /w BulletKit 500 ml
mBMM3368 小鼠骨髓巨噬细胞培养基 Mouse Bone Marrow Macrophage Culture Medium /w BulletKit 500 ml
mKSM5566 小鼠骨髓间充质干细胞培养基 Mouse Bone Marrow Mesenchymal Stem Cell Medium /w BulletKit 500 ml
mBMM3369 小鼠骨髓干细胞培养基 Mouse Bone Marrow Progenitor Cell Medium /w BulletKit 500 ml
mKSM5567 小鼠细胞培养基 Mouse Cell Growth Medium /w BulletKit 500ml
mEPM6621 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml
mEPM6622 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml
mEPM6623 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 250 ml
mDCM7711 小鼠骨髓树突细胞培养基 Mouse Bone marrow-dendritic cells Medium /w BulletKit 500 ml
mESCM8811 小鼠胚胎干细胞培养基 Mouse Embryonic Stem Cell Medium /w BulletKit 500 ml
Chapter 4 CELL GROWTH SUPPLEMENT
1166 内皮细胞生长助生长溶液-ECGS Endothelial Cell Growth Supplement-ECGS 5 ml
1167 内皮细胞助生长溶液-V Endothelial Cell Growth Supplement-V 5 ml
2267 小鼠成纤维细胞生长因子 Mouse Fibroblast Growth Supplement 5 ml
3368 小鼠骨髓巨噬细胞助生长溶液 Mouse Bone Marrow Macrophage Culture Supplement 5ml
3369 小鼠骨髓干细胞助生长溶液 Mouse Bone Marrow Macrophage Growth Supplement 5ml
5566 小鼠骨髓间充质干细胞助生长溶液 Mouse Bone Marrow Mesenchymal Stem Cell Growth Supplement 5 ml
6621 小鼠上皮细胞助生长溶液-I Mouse Epithelial Cell Growth Supplement-ITS 5 ml
6622 小鼠上皮细胞助生长溶液-E Mouse Epithelial Cell Growth Supplement-EGF 5 ml
6911 胎牛血清-2 ml Fetal Bovine Serum (FBS) 2 ml
6912 胎牛血清-20 ml Fetal Bovine Serum (FBS) 25 ml
6913 胰酶/EDTA消化液 (T/E)-50 ml 0.05% Trypsin/EDTA Solution 50 ml
6914 胰酶/EDTA消化液 (T/E)-100 ml 0.05% Trypsin/EDTA Solution 100 ml
6915 非酶细胞裂解液 Enzyme-free Cell Dissociation solution 100 ml
6916 细胞冻存液 Cell Freezing Medium 50 ml
6917 青霉素/链霉素溶液-5 ml Penicillin/Streptomycin Solution 5 ml
6918 青霉素/链霉素溶液-25 ml Penicillin/Streptomycin Solution 25 ml
6919 抗霉菌溶液-25 ml Antibiotic/Antimycotic Solution 25 ml
6920 抗霉菌溶液-5 ml Antibiotic/Antimycotic Solution 5 ml
6921 左旋谷氨酰胺溶液 L-Glutamine-200 mM (100X), liquid 5 ml
6922 非必需氨基酸溶液 Non-Essential Amino Acids Solution 10 mM (100X), liquid 5 ml
6923 丙酮酸钠溶液 Sodium Pyruvate Solution 100 mM (100X), liquid 5 ml
6924 维生素溶液 Vitamin Solution (100X), liquid 5 ml
6925 2-巯基乙醇溶液 2-Mercaptoethanol (1,000X), liquid prepared in D-PBS, 5 ml
6926 胰岛素铁硒传递蛋白 Insulin-Transferrin-Selenium (100X) 5 ml
6927 牛脑垂体提取物 Bovine Pituitary Extract 25 ml
6928 HEPES 缓冲液 HEPES Buffer Solution (1 M) 5 ml
6929 胰酶/EDTA消化液 (T/E)-50 ml 0.25% Trypsin/EDTA Solution 50 ml