Porcine Primary Pulmonary Artery Fibroblasts

Catalog No. P-6074

Suggested Medium: Fibroblast Medium /w Kit - 500 ml

Catalog No. M2267

Custom Order (1-3 weeks)

Product Description

Porcine Primary Pulmonary Artery Fibroblasts from Cell Biologics are isolated from pulmonary artery of porcine. Porcine Primary Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. 

Product Testing

Porcine Primary Pulmonary Artery Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Pulmonary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Porcine Primary Pulmonary Artery Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.

Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics Products

Porcine Primary Pulmonary Artery Fibroblasts from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

 Chapter 3 CELL CULTURE MEDIA 培养基 mECM1166 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml mECM1168 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml mECM1169 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 250 ml mFBM2267 小鼠成纤维细胞培养基 Mouse Fibroblast Growth Medium /w BulletKit 500 ml mBMM3366 小鼠骨髓细胞培养基 Mouse Bone Marrow Leukocyte Culture Medium /w BulletKit 500 ml mBMM3367 小鼠骨髓中性粒细胞培养基 Mouse Bone Marrow Neutrophil Medium /w BulletKit 500 ml mBMM3368 小鼠骨髓巨噬细胞培养基 Mouse Bone Marrow Macrophage Culture Medium /w BulletKit 500 ml mKSM5566 小鼠骨髓间充质干细胞培养基 Mouse Bone Marrow Mesenchymal Stem Cell Medium /w BulletKit 500 ml mBMM3369 小鼠骨髓干细胞培养基 Mouse Bone Marrow Progenitor Cell Medium /w BulletKit 500 ml mKSM5567 小鼠细胞培养基 Mouse Cell Growth Medium /w BulletKit 500ml mEPM6621 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml   mEPM6622 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml  mEPM6623 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit  250 ml mDCM7711 小鼠骨髓树突细胞培养基 Mouse Bone marrow-dendritic cells Medium /w BulletKit 500 ml mESCM8811 小鼠胚胎干细胞培养基 Mouse Embryonic Stem Cell Medium /w BulletKit 500 ml     Chapter 4 CELL GROWTH SUPPLEMENT 1166 内皮细胞生长助生长溶液-ECGS Endothelial Cell Growth Supplement-ECGS 5 ml 1167 内皮细胞助生长溶液-V Endothelial Cell Growth Supplement-V 5 ml 2267 小鼠成纤维细胞生长因子 Mouse Fibroblast Growth Supplement 5 ml 3368 小鼠骨髓巨噬细胞助生长溶液 Mouse Bone Marrow Macrophage Culture Supplement 5ml 3369 小鼠骨髓干细胞助生长溶液 Mouse Bone Marrow Macrophage Growth Supplement 5ml 5566 小鼠骨髓间充质干细胞助生长溶液 Mouse Bone Marrow Mesenchymal Stem Cell Growth Supplement 5 ml 6621 小鼠上皮细胞助生长溶液-I Mouse Epithelial Cell Growth Supplement-ITS 5 ml 6622 小鼠上皮细胞助生长溶液-E Mouse Epithelial Cell Growth Supplement-EGF 5 ml 6911  胎牛血清-2 ml Fetal Bovine Serum (FBS) 2 ml  6912 胎牛血清-20 ml Fetal Bovine Serum (FBS) 25 ml   6913 胰酶/EDTA消化液 (T/E)-50 ml 0.05% Trypsin/EDTA Solution 50 ml   6914 胰酶/EDTA消化液 (T/E)-100 ml 0.05% Trypsin/EDTA Solution 100 ml   6915 非酶细胞裂解液 Enzyme-free Cell Dissociation solution 100 ml   6916 细胞冻存液 Cell Freezing Medium 50 ml   6917 青霉素/链霉素溶液-5 ml Penicillin/Streptomycin Solution 5 ml   6918 青霉素/链霉素溶液-25 ml Penicillin/Streptomycin Solution 25 ml   6919 抗霉菌溶液-25 ml Antibiotic/Antimycotic Solution 25 ml   6920 抗霉菌溶液-5 ml Antibiotic/Antimycotic Solution 5 ml  6921 左旋谷氨酰胺溶液 L-Glutamine-200 mM (100X), liquid 5 ml   6922 非必需氨基酸溶液 Non-Essential Amino Acids Solution 10 mM (100X), liquid  5 ml   6923 丙酮酸钠溶液 Sodium Pyruvate Solution 100 mM (100X), liquid 5 ml   6924 维生素溶液 Vitamin Solution (100X), liquid 5 ml   6925 2-巯基乙醇溶液 2-Mercaptoethanol (1,000X), liquid prepared in D-PBS， 5 ml   6926 胰岛素铁硒传递蛋白 Insulin-Transferrin-Selenium (100X) 5 ml   6927 牛脑垂体提取物 Bovine Pituitary Extract 25 ml   6928 HEPES 缓冲液 HEPES Buffer Solution (1 M) 5 ml   6929 胰酶/EDTA消化液 (T/E)-50 ml 0.25% Trypsin/EDTA Solution 50 ml