ZDFRatDiabetic BrainMicrovascularEndothelialCells

CatalogNo. RD-6023

SuggestedMedium: EndothelialCellMedium/wKit–500ml

CatalogNo. M1266

ProductDescription

ZDFRatDiabeticBrainMicrovascularEndothelialCellsfrom CellBIOLOGics areisolatedfrombraintissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’CultureCompleteGrowthMediumfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶ cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1(CD31)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

ZDFRatDiabetic BrainMicrovascular EndothelialCellsaretestedforexpressionofMarkersusingantibody,PECAM-1Antibody(M-20,sc-1506,SantaCruz)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300,LifeTechnologies)byimmunofluorescencestaining.CellsarealsotestedforuptakeofDil-Ac-LDL(CatalogNo.L-35353,Invitrogen),afunctionalmarkerforendothelialcells.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

ZDFRatDiabetic BrainMicrovascular EndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,transendothelialresistance(TER).StandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

Storageof CellBiologics Products

CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.

AuthorizedUsesof CellBiologics’ Products

ZDFRatDiabetic BrainMicrovascular EndothelialCellsfrom CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.

WarrantyandLiABIlity

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.

 Chapter 3 CELL CULTURE MEDIA 培养基 mECM1166 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml mECM1168 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 500 ml mECM1169 小鼠内皮细胞培养基 Mouse Endothelial Cell Growth Medium /w BulletKit 250 ml mFBM2267 小鼠成纤维细胞培养基 Mouse Fibroblast Growth Medium /w BulletKit 500 ml mBMM3366 小鼠骨髓细胞培养基 Mouse Bone Marrow Leukocyte Culture Medium /w BulletKit 500 ml mBMM3367 小鼠骨髓中性粒细胞培养基 Mouse Bone Marrow Neutrophil Medium /w BulletKit 500 ml mBMM3368 小鼠骨髓巨噬细胞培养基 Mouse Bone Marrow Macrophage Culture Medium /w BulletKit 500 ml mKSM5566 小鼠骨髓间充质干细胞培养基 Mouse Bone Marrow Mesenchymal Stem Cell Medium /w BulletKit 500 ml mBMM3369 小鼠骨髓干细胞培养基 Mouse Bone Marrow Progenitor Cell Medium /w BulletKit 500 ml mKSM5567 小鼠细胞培养基 Mouse Cell Growth Medium /w BulletKit 500ml mEPM6621 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml   mEPM6622 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit 500 ml  mEPM6623 小鼠上皮细胞培养基 Mouse Epithelial Cell Growth Medium /w BulletKit  250 ml mDCM7711 小鼠骨髓树突细胞培养基 Mouse Bone marrow-dendritic cells Medium /w BulletKit 500 ml mESCM8811 小鼠胚胎干细胞培养基 Mouse Embryonic Stem Cell Medium /w BulletKit 500 ml     Chapter 4 CELL GROWTH SUPPLEMENT 1166 内皮细胞生长助生长溶液-ECGS Endothelial Cell Growth Supplement-ECGS 5 ml 1167 内皮细胞助生长溶液-V Endothelial Cell Growth Supplement-V 5 ml 2267 小鼠成纤维细胞生长因子 Mouse Fibroblast Growth Supplement 5 ml 3368 小鼠骨髓巨噬细胞助生长溶液 Mouse Bone Marrow Macrophage Culture Supplement 5ml 3369 小鼠骨髓干细胞助生长溶液 Mouse Bone Marrow Macrophage Growth Supplement 5ml 5566 小鼠骨髓间充质干细胞助生长溶液 Mouse Bone Marrow Mesenchymal Stem Cell Growth Supplement 5 ml 6621 小鼠上皮细胞助生长溶液-I Mouse Epithelial Cell Growth Supplement-ITS 5 ml 6622 小鼠上皮细胞助生长溶液-E Mouse Epithelial Cell Growth Supplement-EGF 5 ml 6911  胎牛血清-2 ml Fetal Bovine Serum (FBS) 2 ml  6912 胎牛血清-20 ml Fetal Bovine Serum (FBS) 25 ml   6913 胰酶/EDTA消化液 (T/E)-50 ml 0.05% Trypsin/EDTA Solution 50 ml   6914 胰酶/EDTA消化液 (T/E)-100 ml 0.05% Trypsin/EDTA Solution 100 ml   6915 非酶细胞裂解液 Enzyme-free Cell Dissociation solution 100 ml   6916 细胞冻存液 Cell Freezing Medium 50 ml   6917 青霉素/链霉素溶液-5 ml Penicillin/Streptomycin Solution 5 ml   6918 青霉素/链霉素溶液-25 ml Penicillin/Streptomycin Solution 25 ml   6919 抗霉菌溶液-25 ml Antibiotic/Antimycotic Solution 25 ml   6920 抗霉菌溶液-5 ml Antibiotic/Antimycotic Solution 5 ml  6921 左旋谷氨酰胺溶液 L-Glutamine-200 mM (100X), liquid 5 ml   6922 非必需氨基酸溶液 Non-Essential Amino Acids Solution 10 mM (100X), liquid  5 ml   6923 丙酮酸钠溶液 Sodium Pyruvate Solution 100 mM (100X), liquid 5 ml   6924 维生素溶液 Vitamin Solution (100X), liquid 5 ml   6925 2-巯基乙醇溶液 2-Mercaptoethanol (1,000X), liquid prepared in D-PBS， 5 ml   6926 胰岛素铁硒传递蛋白 Insulin-Transferrin-Selenium (100X) 5 ml   6927 牛脑垂体提取物 Bovine Pituitary Extract 25 ml   6928 HEPES 缓冲液 HEPES Buffer Solution (1 M) 5 ml   6929 胰酶/EDTA消化液 (T/E)-50 ml 0.25% Trypsin/EDTA Solution 50 ml