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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons/TR-100-FJ/1 Ea
商品描述
| Description | Thecausesandeffectsofneuronaldegenerationareofmajorinteresttoawidevarietyofneuroscientists.Parallelingthisgrowinginterestisanincreasingnumberofmethodsapplicabletothedetectionofneuronaldegeneration.Fluoro-Jade®Cstainsalldegeneratingneuronsregardlessofspecificinsultormechanismofcelldeath.Fluoro-JadeCexhibitsthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalisingnotonlydegeneratingnervecellbodiesbutalsodistaldendrites,axonsandterminals.ThedyeishighlyresistanttofADIngandiscompatIBLewithvirtuallyallhistologicalprocessingandstainingprotocols. |
| Applications | TheFluoro-JadeC"ReadytoDilute"(RTD)StainingKitprovidesaneasytouseassortmentofFluoro-JadeC,DAPI,sodiumhydroxideandpotassiumpermanganateinliquidform.Followingourdetailedprotocol,Fluoro-JadeClabelleddegeneratingneuronsarevisualisedwithbluelightexcitationwhileDAPIcounterstainedcellnucleiarevisualisedwithultra-violetIllumination.TheFluoro-JadeCStainingKitcanbeusedonallkindsofpreservedtissues,includingfresh-frozen,paraformaldehydeorformalinfixed,andformalinfixed,paraffin-embeddedtissues. |
| Comments | MATERIALSPROVIDED SodiumHydroxide,SolutionA(Dilute1:10priortouse)-40mL PotassiumPermanganate,SolutionB(Dilute1:10priortouse)-40mL Fluoro-JadeC,SolutionC(Dilute1:10priortouse)-40mL DAPI,SolutionD(AddtodilutedFluoro-JadeC)-40mL EQUIPMENTANDREAGENTSNEEDED Gelatincoatedmicroscopeslides Stainingdishes/Coplinjars Coverslips DPXmountingmedia Slidewarmer Convectionoven Distilledwater Ethanol Xylene NUMBEROFSLIDESPROCESSED: Theactualnumberofslidesprocessedbythiskitwilldependlargelyuponthevesselthatisusedtoincubatetheslides.IfusingastandardCoplinJar,itscapacityis50mLandtypicallyholds5slidesperjar.Ifusingsuchadevice,then80-100slidesstainedper50mlofworkingsolution(or,5mlofstocksolution)couldbeprocessedinoneday.NotethediluteddyeisNOTstableandwillnotstoreovernight.Itisbesttousefreshlydiluteddyeeachtimeanexperimentalbatchisstarted. FinalworkingconcentrationsofFJC:0.0001% FinalworkingconcentrationofKMnO4:0.06% |
| Specificity | Degeneratingneurons,andneuronaldegeneration.Thereisnospecificstaininginnormalhealthybrain. |
| Cross-reactivity | Note:SomeresearchersundersomeconditionsreportbloodvesselstainingwithFluoroJade.ThismaybebecauseFluoroJadeisananalogueofeosin(whichstainsbloodcells).Ingeneral,goodperfusionandpreparationofthetissueshouldhelppreventbloodvesselstainingbutitmaynotbepossibletoeliminateitentirely.Inourexperienceitisgenerallypossibletodistinguishneuronalfrombloodvesselsstainingbyeye. |
| Form | ThereagentsintheFluoroJadekitareallsuppliedinaliquidformatandareready-to-dilute. |
| Appearance | FJCvisualizationisaccomplishedusingbluelightora488nmLaser.ExcitationPeak:495nmEmissionPeak:521nmFiltersystemforvisualizing:Fluorescein/FITC |
| Reconstitution | Dilutesolutionsasdirectedintheprotocolinstructions.Sometimessmallprecipitatesmaybepresentinthestockordilutedsolutions.Completemixingofthedilutedsolutionsusuallydissolvestheprecipitates.Theprecipitates,ifnotremoved,donotusuallycauseanydifficultiesifthewashingstepsarefollowedasinstructed.Optional:ForentirelycleansolutionsBiosensisrecommendsfilteringthedilutedsolutionthoughethanolandNaOHcompatiblesyringeorvacuumfilterdevicespriortocontactwithtissueslides. |
| Storage | Theunopenedkitcanbestoredforupto6monthsat2-8ºCafterthedateofreceipt.Thekitandcomponentsshouldbestoredprotectedfromlight.DilutedFJCdyesolutionsarenotstableandshouldbeusedwithin4hoursofmaking.Theotherdilutedsolutionscanbereusedandstoredforupto48hoursifrefrigeratedandprotectedfromlight.Bestresultsrequirefreshlydilutedsolutions.Werecommendusingaseptictechniqueswhenhandlingthereagentstoavoidbacterialgrowthandcontamination. TheFJCReadytodilutekitisshippedambientandstableatroomtemperatureduringtransport.Refrigerateuponarrival,donotfreeze. |
| ExpiryDate | Unopenedkit6monthsat2-8ºCprotectedfromlight.SeeStorageinstructionsforworkingsolutionsrecommendations. |
| SpecificReferences | Freitas-AndradeM,SheJ,BechbergerJ,NausCC,SinWC(2017)"Acuteconnexin43temporalandspatialexpressioninresponsetoischemicstroke."JCellCommunSignal.[Epubaheadofprint]Application:IF.Species:Mouse XieZ,EnkhjargalB,WuL,ZhouK,SunC,HuX,GospodarevV,TangJ,YouC,ZhangJH(2017)"Exendin-4attenuatesneuronaldeathviaGLP-1R/PI3K/Aktpathwayinearlybraininjuryaftersubarachnoidhemorrhageinrats."Neuropharmacology.128:142-51Application:IF.Species:Rat KhanD(2017)"TheRelationshipbetweenAstrocytes,InflammationandEpileptogenesis."PhDThesis.Application:IF.Species:Mouse FanLF,HePY,PengYC,DuQH,MaYJ,JinJX,XuHZ,LiJR,WangZJ,CaoSL,LiT,YanF,GuC,WangL,ChenG(2017)"Mdivi-1amelioratesearlybraininjuryaftersubarachnoidhemorrhageviathesuppressionofinflammation-relatedblood-brainbarrierdisruptionandendoplasmicreticulumstress-basedapoptosis."FreeRadicBiolMed.[Epubaheadofprint]Application:IF.Species:Rat 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KononenkoNL,ClaßenGA,KuijpersM,PuchkovD,MaritzenT,TempesA,MalikAR,SkaleckaA,BeraS,JaworskiJ,HauckeV(2017)"RetrogradetransportofTrkB-containingautophagosomesviatheadaptorAP-2mediatesneuronalcomplexityandpreventsneurodegeneration."NatCommun.8:14819Application:IF.Species:Mouse LianYD,ChenZX,ZhuKR,SunSY,ZhuLP(2017)"Effectofequipotentdosesofbupivacaineandropivacaineinhigh‐fatdietfedneonatalrodentmodel."BrazJAnesthesiol.67(2):131-8Application:IF.Species:Rat MamoJCL,LamV,GilesC,CoulsonSH,FimognariN,MooranianA,Al-SalamiH,TakechiR(2017)"Antihypertensiveagentsdonotpreventbloodbrainbarrierdysfunctionandcognitivedeficitsindietary-inducedobesemice."IntJObes(Lond).[Epubaheadofprint]Application:IF.Species:Mouse IwasaK,YamamotoS,YagishitaS,MaruyamaK,YoshikawaK(2017)"Excitotoxicity-inducedprostaglandinD2productioninducessustainedmicroglialactivationanddelayedneuronaldeath."JLipidRes.58(4):649-55Application:IF.Species:Rat XieZ,HuangL,EnkhjargalB,ReisC,WanW,TangJ,ChengY,ZhangJH(2017)"IntranasaladmiNISTrationofrecombinantNetrin-1attenuatesneuronalapoptosisbyactivatingDCC/APPL-1/AKTsignalingpathwayaftersubarachnoidhemorrhageinrats."Neuropharmacology.http://dx.doi.org/10.1016/j.neuropharm.2017.03.025[Inpress]Application:IF.Species:Rat KatayamaY,InabaT,NitoC,SudaS,UedaM(2016)"Neuroprotectiveeffectsofclarithromycinagainstneuronaldamageincerebralischemiaandinculturedneuronalcellsafteroxygen-glucosedeprivation."LifeSci.PMID:27825902[Epubaheadofprint]Application:IF.Species:Rat YangLY,GreigNH,HuangYN,HsiehTH,TweedieD,YuQS,HofferBJ,LuoY,KaoYC,WangJY(2016)"Post-traumaticadministrationofthep53inactivatorpifithrin-αoxygenanaloguereduceshippocampalneuronallossandimprovescognitivedeficitsafterexperimentaltraumaticbraininjury."NeurobiolDis.PMID:27553877[Epubaheadofprint]Application:IF.Species:Rat IzumidaH,TakagiH,FujisawaH,IwataN,NakashimaK,TakeuchiS,IwamaS,NambaT,KomatuY,KaibuchiK,OisoY,ArimaH,SugimurY(2016)"NMDAreceptorantagonistpreventscelldeathinthehippocampaldentategyrusinducedbyhyponatremiaaccompanyingadrenalinsufficiencyinrats."ExpNeurol.PMID:27527984[Epubaheadofprint]Application:IF.Species:Rat DixonBJ,ChenD,ZhangY,FloresJ,MalaguitJ,NowrangiD,ZhangJH,TangJ.(2016)"IntranasalAdministrationofInterferonBetaAttenuatesNeuronalApoptosisviatheJAK1/STAT3/BCL-2PathwayinaRatModelofNeonatalHypoxic-IschemicEncephalopathy."ASNNeuro.PMID:276838778(5)Application:IF.Species:Rat WangJY,HuangYN,ChiuCC,TweedieD,LuoW,PickCG,ChouSY,LuoY,HofferBJ,GreigNH,WangJY(2016)"Pomalidomidemitigatesneuronalloss,neuroinflammation,andbehavioralimpairmentsinducedbytraumaticbraininjuryinrat."JNeuroinflammation.PMID:2735305313(1):168Application:IF.Species:Rat 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KhanD,DupperA,DeshpandeT,GraanPN,SteinhäuserC,BednerP.(2016)"Experimentalfebrileseizuresimpairinterastrocyticgapjunctioncouplinginjuvenilemice."JNeurosciRes.PMID:26931373[Epubaheadofprint]Application:IF.Species:Mouse ByeN,ChristieKJ,TurbicA,BasraiHS,TurnleyAM(2016)"Rhokinaseinhibitionfollowingtraumaticbraininjuryinmicepromotesfunctionalimprovementandacuteneuronsurvivalbuthaslittleeffectonneurogenesis,glialresponsesorneuroinflammation."ExpNeurol.PMID:26896832[Epubaheadofprint]Application:IH.Species:Mouse BednerP,DupperA,HüttmannK,MüllerJ,HerdeMK,DublinP,DeshpandeT,SchrammJ,HäusslerU,HaasCA,HenNEBergerC,TheisM,SteinhäuserC(2015)"Astrocyteuncouplingasacauseofhumantemporallobeepilepsy."Brain.PMID:25765328[Epubaheadofprint]Application:IH4%PFA,vibratome40umsections.Species:Mouse 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RenYY,ZhangHQ,DuanSH,WangSD(2015)"InfluenceofDexmedetomidineonToxicityofIntrathecalKetamineonNeonatalRatSpinalFunction."TropicalJournalofPharmaceuticalResearch.14(3):469-77Application:IH.Species:Rat IseliCE,MerwinWH3rd,Klatt-CromwellC,HutsonKA,EwendMG,AdunkaOF,FitzpatrickDC,BuchmanCA(2014)"EffectofCochlearNerveElectrocauteryontheAdultCochlearNucleus."OtolNeurotol.PMID:25280052[Epubaheadofprint]Application:IH(P) SaitoT,NitoC,UedaM,InabaT,KamiyaF,MuragaK,KatsuraK,KatayamaY(2014)"Continuousoraladministrationofatorvastatinamelioratesbraindamageaftertransientfocalischemiainrats."LifeSci.PMID:2433313394(2):106-14Application:IH.Species:Rat HayashidaK,SanoM,KamimuraN,YokotaT,SuzukiM,OhtaS,FukudaK,HoriS(2014)"Hydrogeninhalationduringnormoxicresuscitationimprovesneurologicaloutcomeinaratmodelofcardiacarrestindependentlyoftargetedtemperaturemanagement."Circulation.PMID:25366995130(24):2173-80Application:IH.Species:Rat |
| ReagentKitprotocol |  TR-100-FJ_January2017_protocol.pdf |
| MSDS | MSDS_TR-100-FJ_as_at_May2017.pdf |
