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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rat Hepatocyte Growth Factor (HGF) ELISA Kit (2 plates)/BEK-2337-2P/1 Ea
商品描述
Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | ForquantitativedetectionofratHGFincellculturesupernatants,serum,andcitrate,orEDTAtreatedplasmasamplesonlywhenusedasdirected | TheratHepatocyteGrowthFactor(HGF)KitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseHGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(alpha)andV496-L728(beta))andtestsamplesareaddedtothewells.Abiotinylateddetectionpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofHGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseHGFinsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Range:156pg/mL-10,000pg/mL;Sensitivity<10 pg/mL BatchNo. | Seeproductlabels | Antigen | Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(alpha)andV496-L728(beta)). | Sequence | Immunogensequence:aminoacids33-495foralphachain,andaminoacids496-728forthebetachainofHGF | OtherNames | Scatterfactor;Hepatopoeitin-A;SF; | Accession | P17945HGF_RAT; | Applications | ELISA | Specificity | NaturalandrecombinantRatHGF | Cross-reactivity | Thereisnodetectablecross-reactivitywithotherrelevantproteins. | ExpiryDate | Unopenedkitstableat4˚Cfor6months,at-20˚Cfor12months. | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/mL-10,000pg/mL | Sensitivity | < 10 pg/mL | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. |
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