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Biosensis/Human Interleukin 10 ELISA Kit (2 plates)/BEK-2045-2P/1 Ea

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¥15940.00
货号:BEK-2045-2P
浏览量:127
品牌:Biosensis
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DescriptionThehumanInterleukin10(IL-10)KitisasandwichELISA.ThecaptureantibodyisamonoclonalhumanIL-10antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownIL-10concentrationareaddedtothesewellsandallowedtocomplexwiththeboundIL-10antibody.AbiotinylatedhumanIL-10monoclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-10presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanIL-10insamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
OtherNamesInterleukin-10;IL-10;Cytokinesynthesisinhibitoryfactor;CSIF;IL10;
AccessionP22301IL10_HUMAN;
SpecificityHumanIL-10
StorageStoreat4°C
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range7.8pg/ml-500pg/mlforcellculturesupernates.3.4pg/ml-250pg/mlforhumanseraandplasma.
Sensitivity< 0.5 pg/ml
KitprotocolClick to download the kit protocolBEK-2045-2P_as_at_Sept2016_protocol.pdf