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商品描述
Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheratInterleukin10(IL-10)KitisasandwichELISA.Thecaptureantibodyisamonoclonalanti-ratIL-10antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownIL-10concentrationareaddedtothesewellsandallowedtocomplexwiththeboundIL-10antibody.Abiotinylatedanti-ratIL-10antibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-10presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratIL-10insamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | OtherNames | Interleukin-10;IL-10;Cytokinesynthesisinhibitoryfactor;CSIF;Il10; | Accession | P29456IL10_RAT; | Specificity | RatIL-10 | Storage | Storeat4øC | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 31.2pg/ml-2,000pg/ml | Sensitivity | < 4 pg/ml | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. |
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