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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
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Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.com| Description | ThemouseVascularendothelialgrowthfactor(VEGF)KitisasandwichELISA.ThecaptureantibodyisaVEGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownVEGFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundVEGFantibody.Abiotinylatedanti-mouseVEGFantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofVEGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseVEGFinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | Vascularendothelialgrowthfactor(VEGF)isadisulfidelinkedhomodimericproteinassociatedwithangiogenesis,vasculogenesisandendothelialcellgrowth.Atleast10isoformsofVEGFareproducedbyalternativesplicingwithvaryinglevelsofexpression.TheisoformsVEGF188,VEGF164andVEGF120arewidelyexpressedwhereastheisoformsVEGF205andVEGF144areuncommon.IsoformsVEGF164andVEGF120arefreelysecretedwhereasisoformVEGF188remainscellassociated. BatchNo. | Seeproductlabels | Antigen | ThestandardandimmunogenusedintheELISAisrecombinantmouseVEGF-Aaminoacids27-190producedinandpurifiedfromaSF21insectcellexpressionsystem. | OtherNames | VEGF-A;VascularpermeABIlityfactor;VPF;VEGFA;VEGF;VEGF-1(VEGF164);VEGF-2(VEGF120) | Accession | Q00731VEGFA_MOUSE; | Specificity | MouseVEGF.Thiskitrecognizesboththe164and120aminoacidresidueformsofmouseVEGF. | Storage | Storeat4°C | SpecificReferences | HuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166. | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/ml | Sensitivity | < 2 pg/ml | Kitprotocol |  BEK-2110-1Plate_as_at_Nov2015protocol.pdf |
