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商品描述
| Description | Saporinisaribosome-inactivatingprotein(RIP)oftypeI.ThismonomericRNAN-glycosidasepurifiedfromseedsoftheplantSaponariaofficinalisalsoknownasSoapwort,iscapableofspecificdepurinationofeukaryoticribosomesthusarrestingproteinsynthesis.NoligandhasbeenidentifiedinsaporinhenceitsinABIlitytotransversethecellmembrane.Duetoitstoxicityandstabilityofthestructure,saporinhasprovenextremelyusefulforconstructionofimmunotoxins.Theexpectedmolecularweightofthepurifiedsaporinis29.5kDa. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Saporin,wholemolecule |
| OtherNames | Saponariaofficinalis;Commonsoapwort |
| Accession | RIP6_SAPOF |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC,Immunofluorescence,ELISA,WesternBlot.Adilutionof1:200to1:2000isrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Confirmedtoreactwithpurifiedsaporin. |
| Cross-reactivity | Nocross-reactivitywithothermoleculeshasbeenreported. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Stirpe,F.etal(1983)BiochemJ216,617-625 2.Tazzari,P.L.etal(1988)CancerImmunolImmunother.26,231-236 3.Strocchi,P.etal(1992)JImmunolMethods.155,57-63 4.Savino,C.etal(2000)FEBSLett.470,239-243 5.Barbieri,L.etal(2000)JBiochem(Tokyo).128,883-889 6.Savino,C.etal(1998)ActaCrystallogrDBiolCrystallogr.54,636-638 7.Santanche,S.etal(1997)BiochemBiophysResCommun.234,129-132 |
