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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Human Stem Cell Factor protein expressed in mammalian cells/PE-1242-5/5 µg
商品描述
| Description | StemCellFactor(SCF)isahematopoieticgrowthfactorthatstimulatestheproliferationofmastcells. SCFalsomediatescell-celladhesionandactssynergisticallywithothercytokines.TheactionsofSCFareexertedbybindingtotheSCFReceptor(PE-1243-50)whichresultsinreceptordimerization.SCFisatypeImembraneglycoproteinwith5potentialN-linkedglycosylationsites.SolubleSCFisproducedbyproteolyticcleavageoftheextracellulardomain. |
| BatchNo. | Seeproductlabel |
| Unitsize | 5µg |
| OtherNames | Kitligand;C-kitligand;Stemcellfactor;SCF;Mastcellgrowthfactor;MGF;KITLG;MGF;SCF; |
| Accession | P21583SCF_HUMAN; |
| Producedin | Human-ADNAsequenceencodingthesignalpeptideandthematureStemCellFactor(SCF)proteinofhumanSCFwasexpressedinmodifiedhuman293cells. |
| MolecularWeight | SCFmigratesasabroadbandbetween20and42kDainSDS-PAGEduetopost-translationalmodifications.UnmodifiedSCFhasapredictedmolecularmassof21.0kDa.SCFseparatesintoanumberofisoformswithapIbetween3.0and7.0in2DPAGEduetopost-translationalmodifications.ThiscompareswiththeunmodifiedSCFwithapredictedpIof5.76. |
| Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
| Biol.activity | TheED50ofSCFistypically10-20ng/mlasmeasuredinacellproliferationassayusingthehumangrowthfactor-dependentM-07ecellline. |
| Form | PurifiedSCFconsistsof0-50%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
| Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
| Storage | Lyophilizedproductsshouldbestoredat2-8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
