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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Human beta Nerve Growth Factor protein expressed in mammalian cells/PE-1239-5/5 µg
商品描述
| Description | Nervegrowthfactor(NGF)isimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons. |
| BatchNo. | Seeproductlabel |
| Unitsize | 5µg |
| OtherNames | Beta-nervegrowthfactor;beta-NGF;NGFB; |
| Accession | P01138NGF_HUMAN; |
| Producedin | Human-ADNAsequenceencodingthehumanbetaNGFproteinsequence(containingthesignalpeptide,pro-peptideandthematurebetaNGFsequence)wasexpressedinmodifiedhuman293cells.Theoreticalpeptidesequence:YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT |
| MolecularWeight | TheNGFproteinmigratesatapproximately12-16kDainSDS-PAGE.Ithasapredictedmolecularmassof13.5kDa.TheproteinseparatesintoanumberofisoformswithapIbetween9and10in2DPAGEduetopost-translationalmodifications.TheunmodifiedproteinhasapredictedpIof9. |
| Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
| Biol.activity | TheED50ofbetaNGFistypically0.2-1.0ng/mlasmeasuredinacellproliferationassayusingthehumangrowthfactordependentTF-1cellline. |
| Form | Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
| Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
| Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
