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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 604-615), intracellular domain [Clone BS292]: IgG/M-
商品描述
| Description | TrkAisamemberoftheneurotrophictyrosinekinasereceptorfamily.Itisamembrane-boundreceptorthatuponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.TrkAisrequiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).TrkAleadstocelldifferentiationsandmayplayaroleinspecifyingsensoryneuronsubtypes.Ithasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating.SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.SUBCELLULARLOCATION:Cellmembrane;single-passtypeImembraneprotein.EndocytosedtotheendosomesupontreatmentofcellswithNGF.ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.BothisoformshavesimilarBIOLOGicalproperties.TISSUESPECIFICITY:IsoformTrkA-IIisprimarilyexpressedinneuronalcells.IsoformTrkA-Iisfoundinnon-neuronaltissues.MutationsinTrkAhavebeenassociatedwithcongenitalinsensitivitytopain,anhidrosis,self-mutalatingbehaviour,mentalretardationandcancer(Reference:www.uniprot.com). |
| BatchNo. | Pleaserefertotheproductlabel. |
| Unitsize | 100µg |
| Antigen | AsyntheticpeptidefromtheintracellularcytoplasmicdomainofhumanTrkA(C-HGPDAKLLAGGE,aa:604-615)hasbeenusedastheimmunogen. |
| Sequence | HGPDAKLLAGGE |
| AntigenLocation | Antigen"speptidesequenceislocatedwithinthecytoplasmicdomainconsistingofamnoacids440796. |
| AntigenLength | 12aminoacids |
| AntibodyType | MousemonoclonalIgG |
| Isotype | IgG3,k-lightchain |
| Clone | BS292 |
| OtherNames | Tropomyosin-relatedkinasereceptor;Highaffinitynervegrowthfactorreceptor;Neurotrophictyrosinekinasereceptortype1;TRK1transformingtyrosinekinaseprotein;p140-TrkA;Trk-A;NTRK1;TRK |
| Accession | Uniprot:P04629;NTRK1_HUMAN |
| Producedin | Mouse |
| MolecularWeight | TrkA140kDa,onSDS-PAGEgels,withisoformsat110kDaandinternalcleavageproductsat41kDa |
| Purity | ProteinGpurifiedmouseimmunoglobulin |
| Applications | WB:Westernblotting:1-3µg/mL,antibodyissuitableforreduced(withDTTorβ-mercaptoethanol)andnon-reducedsamples.Denaturedbutnotreducedsamplesprovidescleanerblotsignalsasdemonstratedinourphotographs. Immunocytochemistry:1-2µg/mL.Antibodyworkson4%formaldehyde-fixedcells.NotethatcellsrequireapermeABIlizationstep,becausetheantibodydetectsacytoplasmicepitopeofTrkA. Otherapplicationsnotyettested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | TrkA.Doesnotcross-reactwithTrkBorTrkC. |
| SpeciesAgainst | Human |
| AntibodyAgainst | Human |
| Cross-reactivity | ReactswithhumanTrkA.Knowntocross-reactwithTrkAfromratandmouse.Expectedtocross-reactwithothermammalianspeciesbasedonpeptideantigensequencesimilarity. |
| Form | LyophilizedfromPBS,pH7.4,containing3%trehalosewithoutpreservatives. |
| Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservatives. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstability.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
| GeneralReferences | 1.CabreraN.etal.,TrkAreceptorectodomaincleavagegeneratesatyrosine-phosphorylatedcell-associatedfrangment.JCellBiol,1996,Vol.132(3),pp.427-436. 2.RossiF.M.etal.,ExpressionofthenervegrowthfactorreceptorsTrkAandp75NTRinthevisualcortexoftherat:developmentandregulationbythecholinergicinput.JNeurosci,Februar1,2002,22(3):912-919. |
