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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG/M-
商品描述
| Description | TrkAisamemberoftheneurotrophictyrosinekinasereceptorfamily.Itisamembrane-boundreceptorthatuponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.TrkAisrequiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).TrkAleadstocelldifferentiationsandmayplayaroleinspecifyingsensoryneuronsubtypes.Ithasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating.SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.SUBCELLULARLOCATION:Cellmembrane;single-passtypeImembraneprotein.EndocytosedtotheendosomesupontreatmentofcellswithNGF.ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.BothisoformshavesimilarBIOLOGicalproperties.TISSUESPECIFICITY:IsoformTrkA-IIisprimarilyexpressedinneuronalcells.IsoformTrkA-Iisfoundinnon-neuronaltissues.MutationsinTrkAhavebeenassociatedwithcongenitalinsensitivitytopain,anhidrosis,self-mutalatingbehaviour,mentalretardationandcancer(Reference:www.uniprot.com). |
| BatchNo. | Pleaserefertotheproductlabel. |
| Unitsize | 100µg |
| Antigen | AsyntheticpeptidefromtheextracellulardomainofhumanTrkA(C-SATVMKSGGLPS,aa:235-246)hasbeenusedastheimmunogen. |
| Sequence | SATVMKSGGLPS |
| AntigenLocation | Peptidesequenceislocatedwithintheextracellulardomainconsistingofaminoacids33-432. |
| AntigenLength | 12aminoacids |
| AntibodyType | MousemonoclonalIgG |
| Isotype | IgG3,k-lightchain |
| Clone | BS470 |
| OtherNames | Tropomyosin-relatedkinasereceptor;Highaffinitynervegrowthfactorreceptor;Neurotrophictyrosinekinasereceptortype1;TRK1transformingtyrosinekinaseprotein;p140-TrkA;Trk-A;NTRK1;TRK |
| Accession | Uniprot:P04629;NTRK1_HUMAN |
| Producedin | Mouse |
| MolecularWeight | GlycosylatedhumanTrkAhasanapparentmolecularweightof140kDa |
| Purity | ProteinGpurifiedmouseimmunoglobulin |
| Applications | Flowcytometry:20µg/mL. Immunocytochemistry:1-5µg/mL. FlowcytometrydataandimmunofluorescencestainingofTrkA(extracellulardomain)receptorinSHSY-5YcellshasshownthatdetectionofTrkAexpressiondependsonitscellularlocalization(membranevs.internalstores).Thus,permeABIlizationofcellsmayberequired,despitethatcloneBS470wasraisedagainsttheextracellulardomainofTrkA. Otherapplicationsnotyettested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | TrkA.Doesnotcross-reactwithTrkBorTrkC. |
| SpeciesAgainst | Human |
| AntibodyAgainst | Human |
| Cross-reactivity | ReactswithhumanTrkA.Whilenottestedyet,M-1723-100isnotexpectedtoreactwithTrkAfromotherspeciesduetoaminoacidsequencedissimilarity. |
| Form | LyophilizedfromPBS,pH7.4,containing3%trehalosewithoutpreservatives. |
| Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservatives. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20°Cforahigherstability.Antibodycontainsnopreservatives.Storeat4°Cwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
