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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to human p75NTR [MLR2] - ATTO 488/M-028-50-AT/50 µg
商品描述
| Description | p75NTRwasoriginallydiscoveredasalowaffinitynervegrowthfactorreceptor.Lateritwasfoundthatitwasthereceptorforallneurotrophins.Itmediatessignalsofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Recently,ithasbeenrevealedthatp75NTRnotonlyactsasthereceptorforneurotrophinsbutalsothereceptorformanyotherpathologicalligandssuchasprions,rABIesvirusandamyloidbeta.p75NTRalsoactsasaco-receptorforNOGOwhichmediatesinhibitorysignalsofmyelinassociatedprotein.p75NTRishighlyexpressedinanumberofnon-neuronalandneuronalcellsincludingmotorneuronsduringdevelopmentandalsoindamagedneurons.FUNCTION:LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells.SUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µg |
| Antigen | Humanrecombinantp75. |
| Isotype | IgG2a |
| Clone | MLR2 |
| OtherNames | Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;Tumornecrosisfactorreceptorsuperfamilymember16;CD271;NGFR;TNFRSF16; |
| Accession | P08138TNR16_HUMAN; |
| Producedin | Mouse |
| Purity | Immunoglobulin(IgG2A)waspurifiedusingProteinGcolumn(AmershamPharmacia),polishedwithSephacryl200HR(AmershamPharmacia)inPBS.TheIgGwasthenconjugatedtoATTO488(ATTOTEC)andpurifiedviagelfiltrationusingaG25mediumgraingel. |
| Applications | Immunofluorescence,flowcytometry.Suggestedworkingdilutions:Forimmunofluorescenceaconcentrationof1-5µg/mlisrecommended.LIGHTfixationisamust,orunfixedworksbest.Epitopeissensitivetofixation.ThisantibodyisnotrecommendedfordenaturingWBapplications.ForFACSaconcentrationof20µg/mlisrecommended.ThisantibodydoesnotblockNeurotrophinbinding.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SpecificitywasdemonstratedbyimmunofluorescenceandFACSanalysis.Immunohistochemicalstainingoflivemotorneuronsinspinalcordfollowinglesionofsciaticnerveandincholinergicneuronsofthebasalforebraincorrespondingtopublishedliterature. |
| Cross-reactivity | Reactswithhuman,mouseandrat.Otherspecieshavenotyetbeentestedbutitisexpectedthatthisantibodywillbeusefulforthestudyofp75inprimatesandotherspecies. |
| Form | Liquid,PBSpH7.4 |
| Storage | Theantibodyconjugatecanbestoredat4ºCforupto4monthswiththeadditionofappropriateantibacterialagent. |
| SpecificReferences | 1.WieseS,HerrmannT,DrepperC,JablonkaS,FunkN,KlausmeyerA,RogersML,RushRA&SendtnerM(2009)Isolationandenrichmentofembryonicmousemotoneuronsfromthelumbarspinalcordofindividualmouseembryos.NatProtoc.2010;5(1):31-8. 2.ChacónPJ,ArévaloMA,TébarAR.(2010)NGF-activatedproteintyrosinephosphatase1BmediatesthephosphorylationanddegradationofI-kappa-BalphacoupledtoNF-kappa-Bactivation,therebycontrollingdendritemorphology.MolCellNeurosci.2010Apr;43(4):384-93. |
| References | 1.RogersML,AtmosukartoI,BerhanuDA,MatusicaD,MacardleP,RushRA(2006).Functionalmonoclonalantibodiestop75neurotrophinreceptorraisedinknockoutmice.NeurosciMethods.158(1)pp.109-120 2.MatusicaD,FenechMP,RogersML,RushRA.(2008)CharacterisationanduseoftheNSC-34celllinerforstudyofnerurotrophinreceptortrafficking.J.Neurosci.Res.86(3)pp.553-65. 3.HuhCY,DanikM,ManseauF,TrudeauLE,WilliamsS.(2008)ChronicexposuretonervegrowthfactorincreasesacetylcholineandglutamatereleasefromcholinergicneuronsoftheratmedialseptumanddiagonalbandofBocaviamechanismsmediatedbup75NTR.J.Neurosci.28(6)pp.1404-9. 4.LagaresA,LiHY,ZhouXF,AvendanoC.(2007)Primarysensoryneuronadditionintheadultrattrigeminalganglion:evidenceforneuralcrestglio-neuronalprecursormaturation.J.Neurosci.27(30)pp.7939-53. |
