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Biosensis/Rabbit polyclonal antibody to human P2X purinoceptor 3 (385-397): Affinity purified/R-1116-100/100 µg

价格
¥5940.00
货号:R-1116-100
浏览量:127
品牌:Biosensis
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Description
Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenAsyntheticpeptide(KQSTDSGAFSIGH)correspondingtoaregion(385-397)fromtheC-terminusofhumanP2Xpurinoceptor3(P2X3).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA.
OtherNamesP2Xpurinoceptor3;P2X3;ATPreceptor;Purinergicreceptor;P2RX3;
AccessionP56373P2RX3_HUMAN;
ProducedinRabbit
PurityAffinitypurifiedonantigencolumn
ApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanP2Xpurinoceptor3hasapredictedlengthof397aminoacidsandMWof44kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.
Cross-reactivityHuman;rat;predictedtoreactwithmouseduetosequencehomology;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3
ReconstitutionReconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase