品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to human nicotinic acetylcholine receptor subunit alpha-1 (22-36): Affinity purified/R-1
商品描述
| Description | Uponbindingofacetylcholine,theacetylcholinereceptorrespondsbyundergoinganextensivechangeofconformationwhichaffectsitsalphaandbetasubunitsandleadstotheopeningofanion-conductingchannelacrosstheplasmamembrane.Atleast2isoformsofthealpha-1subunitareproducedfromalternatesplicing.Isoform1isonlyexpressedinskeletalmusclewhereasisoform2isconstitutivelyexpressedinskeletalmuscle,brain,heart,kidney,liver,lungandthymus. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(EHETRLVAKLFKDYS)correspondingtoaregion(22-36)fromtheN-terminusofhumannicotinicacetylcholinereceptorsubunitalpha-1. |
| OtherNames | Acetylcholinereceptorsubunitalpha;CHRNA1;ACHRA;CHNRA; |
| Accession | P02708ACHA_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | WesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.Humanacetylcholinereceptorsubunitalpha-1(isoform2)hasapredictedlengthof482aminoacidsandMWof55kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;rat;predictedtoreactwithmouseduetosequencehomology; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
