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Biosensis/Rabbit polyclonal antibody to human nicotinic acetylcholine receptor subunit alpha-1 (21-35): Affinity purified/R-1

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¥5940.00
货号:R-1033-100
浏览量:127
品牌:Biosensis
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Description
Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
ReplacedbyR-1123-100
BatchNo.Seeproductlabel
Unitsize100µg
AntigenAsyntheticpeptide(SEHETRLVAKLFKDY)correspondingtoaregion(21-35)fromtheN-terminusofhumannicotinicacetylcholinereceptorsubunitalpha-1.
OtherNamesAcetylcholinereceptorsubunitalpha;CHRNA1;ACHRA;CHNRA;
AccessionP02708ACHA_HUMAN;
ProducedinRabbit
PurityAffinitypurifiedonantigencolumn
ApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.Humanacetylcholinereceptorsubunitalpha-1(isoform2)hasapredictedlengthof482aminoacidsandMWof55kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectacetylcholinereceptorsubunitalpha-1informalinfixedandparaffinembeddedtissues.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.
Cross-reactivityHuman;mouse;rat;rabbit;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilizedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3addedaspreservative
ReconstitutionReconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase