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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to human nicotinic acetylcholine receptor subunit alpha-1 (21-35): Affinity purified/R-1
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Description | Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
Replacedby | R-1123-100 | BatchNo. | Seeproductlabel | Unitsize | 100µg | Antigen | Asyntheticpeptide(SEHETRLVAKLFKDY)correspondingtoaregion(21-35)fromtheN-terminusofhumannicotinicacetylcholinereceptorsubunitalpha-1. | OtherNames | Acetylcholinereceptorsubunitalpha;CHRNA1;ACHRA;CHNRA; | Accession | P02708ACHA_HUMAN; | Producedin | Rabbit | Purity | Affinitypurifiedonantigencolumn | Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.Humanacetylcholinereceptorsubunitalpha-1(isoform2)hasapredictedlengthof482aminoacidsandMWof55kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectacetylcholinereceptorsubunitalpha-1informalinfixedandparaffinembeddedtissues. | Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. | Cross-reactivity | Human;mouse;rat;rabbit; | Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. | Form | Lyophilizedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3addedaspreservative | Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. | Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. | ExpiryDate | 12monthsafterpurchase |
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