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商品描述
| Description | AldolasesareglycolyticenzymethatcatalyzesthereversIBLealdolcleavageoffructose1,6-bisphosphateandfructose-1-phosphatetodihydroxyacetonephosphateandeitherglyceraldehyde3-phosphateorglyceraldehyde,respectively.ThreealdolaseisozymesarefoundinmammalsspecificallyaldolasesA,B,andC,eachofwhichisencodedbyaseparategene.AldolaseAisgenerallyconsideredtobeamuscleenzyme.Northernanalysisofculturedcellssuggeststhatitispresentinbothneuronsandglia(1).AldolaseBisconsideredtobealiver-specificenzymeanditistranscriptionallyactivatedbysignalsfromhormonesanddietaryfactors(2).Intheadult,aldolaseCisthebrain-specificisozyme,withlowbutdetectableactivityinfetaltissues(1,3-6).AldolaseCshares81%aminoacididentitywithaldolaseAand70%identitywithaldolaseB.Earlierstudiesusingisozyme-specificantibodiesreportitslocationingraymatterastrocytesandcellsofthepiamater(5,8).Insituhybridizationofmousecentralnervoussystemusingisozyme-specificprobesrevealedthataldolaseAandCareexpressedincomplementarycelltypes:aldolaseAmRNAisfoundinneurons;aldolaseCmessageisdetectedinastrocytes,somecellsofthepiamater,andPurkinjecells(9).AldolaseCcaninsomesituationsbeusedasanastrocyteMarker.HoweverPurkinjecellsofthecerebellumcontainhighlevelsoftheenzyme,sotheenzymeisnottotallyastrocytespecific. |
| Unitsize | 100ug |
| Antigen | N-terminal20aminoacidsofaldolaseCprotein. |
| AntibodyType | Monoclonal |
| Isotype | IgG1 |
| Producedin | Mouse |
| Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:1,000-1:2,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Theantibodyreactswitha40kDabandbyWesternblotonacrudebovinecerebellumhomogenate.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
| SpeciesAgainst | Theantibodyhasbeendirectlytestedforreactivityinbovine.Itisexpectedthatitwillworkonothermammaltissues. |
| AntibodyAgainst | AldolaseC |
| Form | Lyophilised.Theantibodyhasbeenpurifiedfromtissueculturesupernatant. |
| Appearance | Lyophilised |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| GeneralReferences | 1.PopoviciT,Berwald-NetterY,VibertM,KahnA,SkalaH.LocalizationofaldolaseCmRNAinbraincells.FEBSLett.268,189-193(1990).2.WeberA,MarieJ,CottereauD,SimonM,BesmondC,DreyfusJ.&KahnA.DietaryControlofAldolaseBandL-typePyruvateKinamseRNAsinRat.ÂJ.Biol.Chem259,1798-1802(1984).3.MukaiT,YatsukiH,MasukoS,AraiY,JohK&HoriK.Thestructureofthebrain-specificrataldolaseCgeneanditsregionalexpression.Biochem.Biophys.Res.Commun.174,1035-1042(1991).4.RoydsJ,IronsideJ,WarnaarS,TaylorC&TimperleW.MonoclonalantibodytoaldolaseC:aselectivemarkerforPurkinjecellsinthehumancerebellum.Neuropathol.Appl.Neurobiol.13,11-21(1987).5.ThompsonR.,KynochP.WillsonV.CellularlocalizationofaldolaseCsubunitsinhumanbrain.BrainRes.232,489-493(1982).6.SchapiraF,ReuberM,HatzfeldA.Resurgenceoftwofetal-typeofaldolases(AandC)insomefast-growinghepatomas.Biochem.Biophys.Res.Commun.40,321-327(1970).7.AraiY,KajiharaS,MasudaJ,OhishiS,ZenK,OgataJ.MukaiT.Position-independent,high-level,andcorrectregionalexpressionoftherataldolaseCgeneinthecentralnervoussystemoftransgenicmice.Eur.J.Biochem.221,253-260(1994).8.WachsmuthE,ThornerM.&PfleidererG.Thecellulardistributionofaldolaseisozymesinratkidneyandbraindeterminedintissuesectionsbytheimmuno-histochemicalmethod.Histochemistry,45,143-161(1975).9.WaltherEU,DichgansM,MaricichSM,RomitoRR,YangF,DziennisS,ZacksonS,HawkesR,HerrupK.GenomicsequencesofaldolaseC(ZebrinII)directlacZexpressionexclusivelyinnon-neuronalcellsoftransgenicmice.ProcNatlAcadSciUSA.Mar3;95(5):2615-20(1998). |
