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Biosensis/Rabbit polyclonal antibody to human Fatty acid-binding protein, liver (30-46): Affinity purified/R-1137-100/100 µg

价格
¥6540.00
货号:R-1137-100
浏览量:92
品牌:Biosensis
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商品描述
Description
Fattyacid-bindingprotein,liver(FABP1)isalipidtransportproteinwhichbindslongchainfattyacidsanddeliversthemtotheirreceptorsinthenucleus.FABP1alsobindsbileacids.FABP1isfoundinliverandisasensitiveMarkerforcelldamageoflivercells.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenAsyntheticpeptide(QKGKDIKGVSEIVQNGK)correspondingtoaregion(30-46)fromhumanFattyacid-bindingprotein,liver.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA.
OtherNamesFattyacid-bindingprotein1;L-FABP;FABP1;FABPL;
AccessionP07148FABPL_HUMAN;
ProducedinRabbit
PurityAffinitypurifiedonantigencolumn
ApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanFABP1hasapredictedlengthof127residuesandMWof14kDa.Aconcentrationof2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.
Cross-reactivityHuman;rat;predictedtoreactwithmouseduetosequencehomology;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3
ReconstitutionReconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles
ExpiryDate12monthsafterpurchase