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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to EAAT1/GLAST1 (516-531): Affinity purified/R-1009-100/100 µg
商品描述
Description | Excitatoryaminoacidtransporter1(EAAT1)isaglutamatetransporter(GluT).ItrapidlyremovesL-glutamatefromthesynapticcleftwhichisessentialforterminatingthepostsynapticactionofglutamate.EAAT1isalsoknownashighaffinitysodium-dependentglutamate/aspartatetransporter1(GLAST1).EAAT1ishighlyexpressedincerebellumbutisalsofoundinthefrontalcortex,hippocampusandbasalganglia. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(ENEMKKPYQLIAQDNE)correspondingtoaregion516-531fromtheC-terminusofhumanEAAT1/GLAST1. |
OtherNames | SLC1A3;GLAST;GLT-1; |
Accession | P43003EAA1_HUMAN;P24942EAA1_RAT |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | WesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanEAAT1hasapredictedlengthof542residuesandaMWof60kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;mouse;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilised |
Reconstitution | Eachvialcontains5mgofBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosaland0.05mgNaN3.Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |