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Biosensis/Rabbit polyclonal antibody to EAAT1/GLAST1 (516-531): Affinity purified/R-1009-100/100 µg

价格
¥6540.00
货号:R-1009-100
浏览量:127
品牌:Biosensis
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全国联保
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正规发票
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商品描述
Description
Excitatoryaminoacidtransporter1(EAAT1)isaglutamatetransporter(GluT).ItrapidlyremovesL-glutamatefromthesynapticcleftwhichisessentialforterminatingthepostsynapticactionofglutamate.EAAT1isalsoknownashighaffinitysodium-dependentglutamate/aspartatetransporter1(GLAST1).EAAT1ishighlyexpressedincerebellumbutisalsofoundinthefrontalcortex,hippocampusandbasalganglia.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenAsyntheticpeptide(ENEMKKPYQLIAQDNE)correspondingtoaregion516-531fromtheC-terminusofhumanEAAT1/GLAST1.
OtherNamesSLC1A3;GLAST;GLT-1;
AccessionP43003EAA1_HUMAN;P24942EAA1_RAT
ProducedinRabbit
PurityAffinitypurifiedonantigencolumn
ApplicationsWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanEAAT1hasapredictedlengthof542residuesandaMWof60kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen.
Cross-reactivityHuman;mouse;rat;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilised
ReconstitutionEachvialcontains5mgofBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosaland0.05mgNaN3.Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase