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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit antibody to Clathrin (619-638): whole serum/R-165-100/100 µl
商品描述
| Description | Clathrinisamajorproteincomponentofthepolyhedralcoatofcoatedpitsandvesicles.Twodifferentadapterproteincomplexeslinktheclathrinlatticetoeithertheplasmamembraneorthetrans-Golginetwork.Thesespecialisedorganellesareinvolvedintheintracellulartraffickingofreceptorsandendocytosisofavarietyofmacromolecules.Clathrintriskelions,composedof3heavychainsand3lightchains,arethebasicsubunitsoftheclathrincoat.Inthepresenceoflightchains,hubassemblyisinfluencedbyboththepHandtheconcentrationofcalcium.Clathrinlocalisestothecytoplasmicfaceofcoatedpitandvesiclemembranes. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(KAGLLQRALEHFTDLYDIKR)correspondingtotheaminoacids619-638ofratclathrinconjugatedtodiphtheriatoxinhasbeenusedastheimmunogen.Thepeptideishomologouswiththecorrespondingsequencederivedfromclathrinproteininmouse,humanandbovine. |
| OtherNames | Clathrinheavychain;Clathrinheavychain1;CLH-17;CLTC |
| Accession | CLH_RAT |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC,WB.Thisantibodyworksinimmunohistochemistryonfrozenorparaffinembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilutionsforroutineimmunohistochemistryare1:100to1:4000dependingontissueanddetectionmethod.Itisusualtouseapproximatelya10-foldincreaseforconfocalmicroscopyi.e.adilutionrangeof1:100to1:400.Forwesternblottingadilutionrangeof1:1000to1:8000isrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Thisantibodyhasbeenshowntobespecificforclathrin. |
| Cross-reactivity | Rat |
| Blastit | Ifyouwouldliketousethisproductinanotherspeicesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent. |
| References | 1.KirchhausenT,etal.Proc.Natl.Acad.Sci.U.S.A.84:8805-8809(1987). 2.TerHaarE,etal.Cell95:563-573(1998). |
