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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit antibody to AP-1 complex subunit gamma-1 (5-24): whole serum/R-166-100/100 µl
商品描述
| Description | Adaptinsareimportantcomponentsofclathrin-coatedvesiclestransportingligand-receptorcomplexesfromtheplasmamembraneorfromthetransGolginetworktolysosomes. Togetherwithmediumandsmallsubunits,adaptinsformaheterotetramericcomplexcalledanadaptorwhoseroleistopromotetheformationofclathrin-coatedpitsandvesiclesandtorecognisesortingsignalswithinthecytosolictailsoftransmembranecargomolecules.Gamma-adaptinproteinbelongstotheadaptorproteincomplex1thatplaysaroleinproteinsortinginthelate-Golgi/trans-Golginetworkand/orendosomes.SUBCELLULARLOCATION:Golgiapparatus.Cytoplasmicvesicle,clathrin-coatedvesiclemembrane. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(IRLRELIRTIRTARTQAEER)correspondingtotheaminoacids5-24ofhumanAP-1complexsubunitgamma-1conjugatedtodiphtheriatoxinhasbeenusedastheimmunogen.ThepeptideishomologouswiththecorrespondingsequencederivedfromAP-1complexsubunitgamma-1proteininmouse,rat,dogandzebrafish. |
| Sequence | Humanandratsequencesareidentical. |
| OtherNames | AP-1complexsubunitgamma-1;Adapter-relatedproteincomplex1subunitgamma-1;Gamma1-adaptin;AdaptorproteincomplexAP-1subunitgamma-1;GolgiadaptorHA1/AP1adaptinsubunitgamma-1;Clathrinassemblyproteincomplex1gamma-1largechain;AP1G1;ADTG;CLAPG1 |
| Accession | AP1G1_HUMAN |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC,WB.Thisantibodyworksinimmunohistochemistryonfrozenorwaxembeddedtissue.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilution:Lightmicroscopy1/500to1/2000dependingontissueanddetectionmethod;Immunofluorescence1/50to1/200;Westernblotting1/1000to1/4000isrecommendeddependingondetectionmethod.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThisantibodyhasbeenshowntobespecificforAP-1 |
| Cross-reactivity | Ratandhuman |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeciesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | 0.1mlfilteredlyophilisedserumcontaining0.02%thimerosal. |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.PeyrardM,etal.Genomics50:275-280(1998). 2.TakatsuH,etal.J.Biol.Chem.273:24693-24700(1998). |
