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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit Polyclonal antibody to Glyceraldehyde 3-Phosphate Dehydrogenase/R-1701-100/100 µL
商品描述
| Description | Glyceraldehyde3-PhosphateDehydrogenase(GAPDH)isametabolicenzymeresponsIBLeforcatalyzingonestepintheglycolyticpathway,thereversibleoxidativephosphorylationofglyceraldehyde3-phosphate.GAPDHmayhaveotherrolesintheactivationoftranscriptionandintheregulationofapoptosisaswellasAlzheimer"sdiseaseandHuntington"sdisease.TheimmunogenusedtoraisethisparticularantibodywasextensivelypurifiedpigGAPDH.ThisantibodycanbeusedasaloADIngcontrolforwesternblottingexperiments,allowingcomparisonbetweenthelevelofthisproteinandothersinacellortissue. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µL |
| Antigen | PurifiedpigGAPDH |
| AntibodyType | Polyclonal |
| OtherNames | Glyceraldehyde-3-phosphatedehydrogenase;GAPDH;GAPD;G3PDH;GPDH |
| Accession | P04406G3P_HUMAN;P00355G3P_PIG; |
| Producedin | Rabbit |
| Applications | WesternBlotting(WB)andImmunocytochemistry(ICC).Adilutionof1:10,000-1:30,000isrecommendedforWB.HumanGAPDHhasapredictedlengthof335residuesandaMWof36kDa.Adilutionof1:1,000-1:10,000isrecommendedforICC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Specificforthe~36-38kDaGlyceraldehyde3-PhosphateDehydrogenase(GAPDH)protein. |
| SpeciesAgainst | Human,Rat,Mouse,Bovine.Itisexpectedthatitwillworkonothermammaltissues. |
| BlastURL | Clickhere |
| Form | Lyophilisedrabbitserumwith0.5%sodiumazide. |
| Appearance | Whitepowder |
| Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| GeneralReferences | 1.MorgeneggG,WinklerGC,HubscherU,HeizmannCW,MousJ,KuenzleCC.Glyceraldehyde-3-phosphatedehydrogenaseisanonhistoneproteinandapossibleactivatoroftranscriptioninneurons.JNeuRochem.47:54-6219862.SchulzeH,SchulerA,StuberD,DobeliH,LangernH&HuberG.Ratbrainglyceraldehyde-3-phosphatedehydrogenaseinteractswiththerecombinantcytoplasmicdomainofAlzheimer"sbeta-amyloidprecursorprotein.JNeurochem.60:1915-2219933.BurkeJR,EnghildJJ,MartinME,JouY-S,MyersRM,RosesAD,VanceJM&StrittmatterWJ.HuntingtinandDRPLAproteinsselectivelyinteractwiththeenzymeGAPDH.NatureMed.2:347-350,1996.4.DastoorZ.&Dreyer,J-L.Potentialroleofnucleartranslocationofglyceraldehyde-3-phosphatedehydrogenaseinapoptosisandoxidativestress.J.CellSci.114:1643-16532001.100:429-36(2007). |
