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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to human Insulin receptor subunit alpha (59-75): Affinity purified/R-1124-100/100 µg
商品描述
| Description |  TheInsulinreceptorbindsinsulinandhasatyrosine-proteinkinaseactivity.Thereceptorisatetramerof2alphaand2betasubunitslinkedbydisulfidebonds.Thealphasubunit(135kDa)isextracellularandcontributestotheformationoftheligand-bindingdomainwhereasthebetasubunit(95kDa)containsthekinasedomainandatransmembranedomain.AtleasttwoisoformsoftheInsulinreceptorareproducedbyalternativesplicing. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(HLQILLMFKTRPEDFRD)correspondingtoaregion(59-75)fromhumanInsulinreceptor(isoformLong).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
| OtherNames | IR;EC2.7.10.1;CD220;INSR; |
| Accession | P06213INSR_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurifiedonantigencolumn |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanInsulinreceptor(isoformLong)hasapredictedlengthof1382aminoacidsandMWof156kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Boilingtheparaffinsectionsin10mMcitratebuffer,pH6.0for20minutesisrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;dog;rat;predictedtoreactwithmouseduetosequencehomology; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityforupto6months.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchaseunopened |
