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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to mouse elF2c (Ago2) protein (845-860): Affinity purified/R-1556-100/100 µl
商品描述
| Description | TheelF2c(Argonaute,Ago)proteinisacoreproteinoftheRNA-inducedsilencingcomplex(RISC). |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptidecorrespondingtoasequence(845-860aa)fromtheC-terminusofmouseelF2CconjugatedtoKLH. |
| Sequence | C-KAVQVHQDTLRTMYFA |
| AntigenLocation | 845-860aa(eIF-2C2) |
| OtherNames | Proteinargonaute-1;Proteinargonaute-2;Proteinargonaute-3;Argonaute1;Argonaute2;Argonaute3;mAgo1;mAgo2;Eukaryotictranslationinitiationfactor2C;eIF-2C2;eIF-2C1;eIF2C2;Piwi/argonautefamilyproteinmeIF2C2Proteinslicer;Eif2c2;Ago1;Ago2; |
| Accession | Q8CJG0AG02_MOUSE; |
| Producedin | Rabbit |
| Applications | WesternBlotting(WB),Immunochemistry(IHC)andImmunoprecipitation(IP).TherecommendedconcentrationforIHCinformalinfixedandparaffinembeddedtissuesandformalin/acetonefixedtissuesis1:200-1:500.ForWB,therecommendedconcentrationis1:1,000to1:2,000.TheelFC2proteinhasapredictedlengthof860residuesandtheMWofthemonomeris94kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | SpecificityhasbeenconfirmedbyIHC,WBandIPinmousebrain.ThisantibodyisexpectedtorecogniseatleastelF2c-1,2and3sincetheyhaveidenticalC-termini. |
| Cross-reactivity | Human;mouse;rat;TheAgoproteinsarehighlyconservedsocross-reactivitywithotherspeciesisexpected. |
| BlastURL | Clickhere |
| Form | LyophilisedfromPBS |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | HuangKL,ChadeeAB,ChenCY,ZhangY,ShyuAB.(2013)"PhosphorylationatintrinsicallydisorderedregionsofPAM2motif-containingproteinsmodulatestheirinteractionswithPABPC1andinfluencesmRNAfate."RNA.2013Jan22.[Epubaheadofprint]. LugliG.etal(2008)ExpressionofmicroRNAsandtheirprecursorsinsynapticfractionsofadultmouseforebrain.JNeuRochem.Jul;106(2):650-61. LugliG.etal(2005)DicerandeIF2careenrichedatpostsynapticdensitiesinadultmousebrainandaremodifiedbyneuronalactivityinacalpain-dependentmanner.JNeurochem.Aug;94(4):896-905. |
