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Biosensis/Mouse monoclonal antibody to M-phase inducer phosphatase 3 [IMD-25]: IgG/M-1184-100/100 µg

价格
¥6540.00
货号:M-1184-100
浏览量:116
品牌:Biosensis
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商品描述
DescriptionM-phaseinducerphosphatase3,alsoknownasCDC25C,isatyrosinephosphataseexpressedpredominantlyinG2phase.ItdirectlydephosphorylatesCDC2andtriggersentryintomitosis.M-phaseinducerphosphatase3isalsothoughttosuppressp53-inducedgrowtharrest.Atleast5isoformsareproducedbyalternativesplicing.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenRecombinanthumanM-phaseinducerphosphatase3
CloneIMD-25
OtherNamesDualspecificityphosphataseCdc25C;CDC25C;MPIP3;
AccessionP30307MPIP3_HUMAN;
ProducedinMouse
PurityIgG
ApplicationsWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanM-phaseinducerphosphatase3(isoform1)hasapredictedlengthof473residuesandMWof53kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen.
Cross-reactivityHuman;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3
ReconstitutionReconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial.
StorageAtleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles
ExpiryDate12monthsafterpurchase