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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to M-phase inducer phosphatase 3 [IMD-25]: IgG/M-1184-100/100 µg
商品描述
Description | M-phaseinducerphosphatase3,alsoknownasCDC25C,isatyrosinephosphataseexpressedpredominantlyinG2phase.ItdirectlydephosphorylatesCDC2andtriggersentryintomitosis.M-phaseinducerphosphatase3isalsothoughttosuppressp53-inducedgrowtharrest.Atleast5isoformsareproducedbyalternativesplicing. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | RecombinanthumanM-phaseinducerphosphatase3 |
Clone | IMD-25 |
OtherNames | DualspecificityphosphataseCdc25C;CDC25C;MPIP3; |
Accession | P30307MPIP3_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | WesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanM-phaseinducerphosphatase3(isoform1)hasapredictedlengthof473residuesandMWof53kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |