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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to Vimentin [VMT-24]: IgG/M-1161-100/100 µg
商品描述
| Description | Vimentinsareclass-IIIintermediatefilamentsspecifictomesenchymaltissue.VimentinisanimportantcytoskeletalcomponentresponsIBLeformaintainingcellintegrityandhasaprobableroleintheintracellulartransportofproteinssuchaslipoproteinsbetweenthenucleusandplasmamembrane.ImmunohistochemicalstainingforVimentinischaracteristicofsarcomas. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Pigeyelensvimentin |
| Clone | VMT-24 |
| OtherNames | VIM; |
| Accession | P08670VIME_HUMAN;P02543VIME_PIG; |
| Producedin | Mouse |
| Purity | IgG |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.Humanvimentin(precursor)hasapredictedlengthof466residuesandMWof54kDa.Aconcentrationof1.5-2.5µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
| Cross-reactivity | Human;mouse;rat; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
| Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
