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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Mouse monoclonal antibody to proNGF (30-41), [Clone BS312] - Biotinylated/M-1778-50-B/50 µg
商品描述
| Description | Nervegrowthfactor(NGF)issynthesizedasaprecursor(proNGF)whichmaybereleasedandhavephysiologicalfunctionstocausecelldeath.Itbindsneurotrophinreceptorp75andsortilinandmayalsobeimportantforthedevelopmentofnervoussystem.proNGFissynthesizedintargettissuesandglia,transportedretrogradelyandmaybereleased. BiosensisnowoffersbiotinylatedproNGFantibodyallowingmoreflexibilityinexperimentaldesignbyusingthebiotin-avidin/streptavidindetectionmethod.TheABIlityofbiotinylatedproNGFantibodytodetectproNGFhasbeenvalidatedbyWB. |
| Relatedproducts | Purified,non-biotinylatedproNGFantibody,cat#M-1738-100 |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µg |
| Antigen | Asyntheticpeptide(C-HTIPQAHWTKLQ,aa:30-41)ofhumanproNGFproteinhasbeenusedastheimmunogen.Thesequenceislocatedonthepro-domainoftheproNGFfull-lengthproteinandis80%homologoustomouseandratproNGF. |
| AntibodyType | MousemonoclonalIgGantibody |
| Isotype | IgG2b,lamBDa-lightchain |
| Clone | BS312 |
| OtherNames | Pro-brainnervegrowthfactor;proNGF;NGF |
| Accession | NGF_HUMAN |
| Producedin | Mouse |
| Purity | AntibodywaspurifiedfromcellculturesupernatantbyProteinGchromatography,biotinylatedandbuffer-exchangedintoPBS,pH7.4buffer |
| Applications | ThebiotinylatedproNGFantibodyhasbeentestedbyWesternBlotting(0.1-0.5µg/mL)andisalsoexpectedtoworkinapplicationsvalidatedfortheunlabelledantibody(M-1738-100)atsameorhigherdilutions:FlowCytometryandImmunofluorescence. Otherapplicationsnotyettested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Human |
| Cross-reactivity | Speciescross-reactivitynottested. |
| Conjugate | Biotin |
| Form | LyophilisedfromasolutioncontainingPBSbufferpH7.4with3%trehalose,withoutpreservatives. |
| Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein50µLofsterile,ultrapurewatertogiveaconcentrationof1mg/mL.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservative. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstability.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened |
