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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Neurotrophin Antibody Kit/KIT-NT-500/5 vials, each containing 500 μg sheep IgG
商品描述
| Description | Neurotrophinsaregrowthfactorsthatregulateneuronaldevelopment,maintenanceandplasticity.Thesehomo-dimericproteinsactivateamultitudeofintracellularsignallingcascadesthroughtheirinteractionwithTrkreceptorsandp75NTR. TheNeurotrophinAntibodyKit(TrialSize)containsacollectionofBiosensispopularsheepantibodiesraisedagainstBDNF(S-015-500),NGF(S-052-500),NT3(S-057-500)andNT4/5(S-059-500).ThekitiscompletedwithonevialofnormalsheepIgG(S-1754-100)foruseasnegativecontrol.ThisfullsizeversionofBiosensisNeurotrophinAntibodyKitcontains500μgsheepIgGofeachantibody. Thiskitpresentsacost-effectivewaytoinvestigateneurotrophinproteinexpressionandfunctionbyimmunologicaltechniquessuchasimmunohistochemistry,ELISA,andinhibitionofBIOLOGicalactivity. |
| Relatedproducts | S-015-500,SheeppolyclonalantibodytorhBDNF S-052-500,SheeppolyclonalantibodytobetaNGF S-057-500,SheeppolyclonalantibodytorhNT3 S-059-500,SheeppolyclonalantibodytorhNT4/5 S-1754-500,NormalsheepIgGfromnon-immunizedanimal |
| BatchNo. | Seeproductlabel. |
| Unitsize | 5vials,eachcontaining500μgsheepIgG |
| Antigen | BDNF:recombinanthumanBDNF NGF:nativemousebetaNGFpurifiedfromsuBMAxillarysalivarygland(95%puritybyPAGE) NT3:recombinanthumanNT3 NT4/5:recombinanthumanNT4 |
| AntibodyType | Polyclonal. |
| Isotype | IgG |
| Accession | P23560BDNF_HUMAN; P01139NGF_MOUSE; P20783NTF3_HUMAN; P34130NTF4_HUMAN; |
| Producedin | Sheep. |
| Purity | ProteinGpurifiedIgG. |
| Applications | Immunohistochemistry:1-10µg/mL.BDNFantibodyworksbestonZamboni"sfixed,frozentissue,andisnotrecommendedforparaffin-embeddedtissue. ELISA:1-10µg/mL. WesternBlotting:1-10µg/mL.WesternblottingisnotrecommendedfortheBDNFantibody. Inhibitionofbiologicalactivityinvitro/invivo:ExpectedED50(invivo)valuesareintherangeof2-10µg/mL.TheNGFantibodycompletelyinhibitsneuronalsurvivalandtheoutgrowthactionsofmurineNGFinchickenDRGinvitro. Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.Thenegativecontrol(normalsheepIgG)shouldbeusedatthesameconcentrationastheneurotrophinantibodies. |
| Specificity | BDNFAntibody:Across-reactivityoflessthan1%againstmouseNGF,recombinanthumanNT3orNT4/5hasbeenshownbyELISA. NGFAntibody:Across-reactivityoflessthan1%againstrecombinanthumanBDNF,NT3andNT4/5byELISAhasbeenshown. NT3Antibody:Across-reactivityoflessthan1%tomouseNGF,recombinanthumanBDNFand5%toNT4/5hasbeenshownbyELISA. NT4/5Antibody:Lessthan1%cross-reactivityagainstNGF,recombinanthumanBDNFand5%toNT3hasbeenshownbydotblot. |
| SpeciesAgainst | Human(BDNF,NT3andNT4/5). Mouse(NGF). |
| Cross-reactivity | Neurotrophinssharealargedegreeofhomologyamonghuman,rodentandothermammalianspecies,thustheseneurotrophinantibodiesareusefulforanumberofspecies,includinghuman,mouseandrat. |
| Form | LyophilizedfromPBS,pH7.4,withoutpreservatives. |
| Appearance | Whitepowder. |
| Reconstitution | Reconstituteeachvialwith500µLsterile-filtered,ultrapurewater,toachieveanantibodyconcentrationof1mg/mL.Centrifugebrieflytoremoveanyinsolublematerial. |
| Storage | Storelyophilised,unopenedvialsat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase(unopenedvials). |
