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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit polyclonal antibody to Nicastrin, N-terminal domain: IgG/R-1686-500/500 ug
商品描述
| Description | Nicastrin,atype1membraneglycoprotein,isanessentialcomponentofthegammasecretasecomplexwhichiscriticalforthecleavageoftheamyloidprecursorproteinandothermembraneproteins.Nicastriniswidelyexpressedindifferenttissuetypes.ThisantibodydetectsallprocessedformsofNicastrin. |
| BatchNo. | Seeviallabel |
| Unitsize | 500ug |
| Antigen | Asyntheticpeptide(RGNSVERKIYIPL-C)correspondingtohumanNicastrin[32-44]intheN-terminalregionconjugatedviaadditionalC-terminalCystoDiphtheriatoxoid. |
| AntibodyType | Polyclonal |
| Producedin | Rabbit |
| Applications | WBandIP.Suggestedconcentrationof3-10ug/mLisrecommendedforWB.Humanormousebrainsamplescommonlypreparedwithreducing(50mMDTT),urea(2.3M),SDS(1.5%)in62.5mMTris-HCLpH6.8samplebuffer(withoutboiling)heatingto50Cfor15min.UnprocessedfulllengthhumanNicastrinis709aminoacids,howeverthisproteincontainsanN-terminalsignalpeptidewhichisconsideredtoundergocleavageduringprocessingandtransittothecellplasmamembrane,inadditiontheproteinundergoesglycosylationtoproduceaglycoproteinofabout145kDaapparentMWbySDSPAGE.Biosensisrecommendsthattheoptimalworkingdilutionshouldbedeterminedbytheenduser. |
| Specificity | ConfirmedbyWBusingpeptideabsorption. |
| SpeciesAgainst | ConfirmedbyWBusingpeptideabsorption. |
| Form | LyophilizedfromPBS,pH7.4.Containsnopreservative. |
| Reconstitution | Reconstitutein500µLofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Shorttermstorageat2-8°Cforoneweek.At-20°Casanundilutedliquidforupto12months. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Ilaya,N.T.,Evin,G.,Masters,C.L.,andCulvenor,J.G.(2004)Nicastrinexpressioninmouseperipheraltissuesisnotco-ordinatedwithPresenilinandishighinmuscle.J.NeuRochem.91,230-237. |
