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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Rabbit antibody to Parkin (295-311): whole serum/R-114-100/100 µl
商品描述
| Description | FUNCTION:FunctionswithinamultiproteinE3ubiquitinligasecomplex,catalyzingthecovalentattachmentofubiquitinmoietiesontosubstrateproteins.ThesesubstratesincludeSYT11,CCNE1,GPR37,STUB1,a22kDaO-linkedglycosylatedisoformofSNCAIPandSEPT5.Mayplayamoregeneralroleintheubiquitinproteasomalpathwaybyparticipatingintheremovaland/ordetoxificationofabnormallyfoldedordamagedprotein.LossofthisubiquitinligaseactivityappearstobethemechanismunderlyingpathogenesisofPARK2.Mayprotectneuronsagainstalphasynucleintoxicity,proteasomaldysfunction,GPR37accumulation,andkainate-inducedexcitotoxicity.Mayplayaroleincontrollingneurotransmittertraffickingatthepresynapticterminalandincalcium-dependentexocytosis.RegulatescyclinEduringneuronalapoptosis.Mayrepresentatumorsuppressorgene.SUBCELLULARLOCATION:Cytoplasm.Co-localizeswithSTY11inneutrites.Co-localizeswithSNCAIPinbrainstemLewybodies.TISSUESPECIFICITY:Highlyexpressedinthebrainincludingthesubstantianigra.Expressedinheart,testisandskeletalmuscle.Expressionisdown-regulatedorabsentintumorbiopsies,andabsentinthebrainofPARK2patients.Overexpressionprotectsdopamineneuronsfromkainate-mediatedapoptosis. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(NSLIKELHHFRILGEEQ)aspartofhumanParkinconjugatedtoKLHhasbeenusedastheimmunogen. |
| OtherNames | UbiquitinE3ligasePRKN;Parkinsonjuvenilediseaseprotein2;Parkinsondiseaseprotein2;PARK2;PRKN |
| Accession | PRKN2_HUMAN |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC,WB.Adilutionof1:1000isrecommendedforimmunohistochemistryand1:2000forwesternblot.Nicestainingisachievedinneuronalandcytoplasmicgranulessectionstreatedwithcitratebufferforantigenretrieval.FewinclusionsarestainedbutthesewerenotpositivelyidentifiedasLewybodies.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThisantiserumisknowntobehighlyspecificforParkinshownbyIHCandWB. |
| Cross-reactivity | ThisantibodyisknowntoreactwithratandhumanParkin. |
| Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | 1.SongY.J.C.etal(2009).Degenerationindifferentparkinsoniansyndromesrelatestoastrocytetypeandastrocyteproteinexpression.J.Neuropathol.Exp.Neurol.Oct2009;68(10):1073-1083 2.HuangY.etal(2008).LRRK2andparkinimmunoreactivityinmultiplesystematrophyinclusions.ActaNeuropathol.2008Dec;116(6):639-46. |
| References | 1.Hottorietal.(1998)BiochemBiophysResCommun.249(3):754-758 2.Kitadaetal.(1998).Nature.392:605-608 3.Kumru,H.,etal.,Ann.Neurol.56(4):599-603(2004). 4.Pigullo,S.,etal.,ParkinsonismRelat.Disord.10(6):357-362(2004). 5.Yao,D.,etal.,Proc.Natl.Acad.Sci.U.S.A.101(29):10810-10814(2004). 6.West,A.B.,etal.,J.Biol.Chem.279(28):28896-28902(2004). 7.Wang,F.,etal.,GenesChromosomesCancer40(2):85-96(2004). |
