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Biosensis/Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit for identifying Degenerating Neurons/TR-100-FJ/1 Ea

价格
¥8340.00
货号:TR-100-FJ
浏览量:127
品牌:Biosensis
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商品描述
DescriptionThecausesandeffectsofneuronaldegenerationareofmajorinteresttoawidevarietyofneuroscientists.Parallelingthisgrowinginterestisanincreasingnumberofmethodsapplicabletothedetectionofneuronaldegeneration.Fluoro-Jade®Cstainsalldegeneratingneuronsregardlessofspecificinsultormechanismofcelldeath.Fluoro-JadeCexhibitsthegreatestsignaltobackgroundratio,aswellasthehighestresolution.Thistranslatestoastainofmaximalcontrastandaffinityfordegeneratingneurons.Thismakesitidealforlocalisingnotonlydegeneratingnervecellbodiesbutalsodistaldendrites,axonsandterminals.ThedyeishighlyresistanttofADIngandiscompatIBLewithvirtuallyallhistologicalprocessingandstainingprotocols.
ApplicationsTheFluoro-JadeC"ReadytoDilute"(RTD™)StainingKitprovidesaneasytouseassortmentofFluoro-JadeC,DAPI,sodiumhydroxideandpotassiumpermanganateinliquidform.Followingourdetailedprotocol,Fluoro-JadeClabelleddegeneratingneuronsarevisualisedwithbluelightexcitationwhileDAPIcounterstainedcellnucleiarevisualisedwithultra-violetIllumination.TheFluoro-JadeCStainingKitcanbeusedonallkindsofpreservedtissues,includingfresh-frozen,paraformaldehydeorformalinfixed,andformalinfixed,paraffin-embeddedtissues.
CommentsMATERIALSPROVIDED

SodiumHydroxide,SolutionA(Dilute1:10priortouse)-40mL
PotassiumPermanganate,SolutionB(Dilute1:10priortouse)-40mL
Fluoro-JadeC,SolutionC(Dilute1:10priortouse)-40mL
DAPI,SolutionD(AddtodilutedFluoro-JadeC)-40mL


EQUIPMENTANDREAGENTSNEEDED

Gelatincoatedmicroscopeslides
Stainingdishes/Coplinjars
Coverslips
DPXmountingmedia
Slidewarmer
Convectionoven
Distilledwater
Ethanol
Xylene

NUMBEROFSLIDESPROCESSED:

Theactualnumberofslidesprocessedbythiskitwilldependlargelyuponthevesselthatisusedtoincubatetheslides.IfusingastandardCoplinJar,itscapacityis50mLandtypicallyholds5slidesperjar.Ifusingsuchadevice,then80-100slidesstainedper50mlofworkingsolution(or,5mlofstocksolution)couldbeprocessedinoneday.NotethediluteddyeisNOTstableandwillnotstoreovernight.Itisbesttousefreshlydiluteddyeeachtimeanexperimentalbatchisstarted.

FinalworkingconcentrationsofFJC:0.0001%
FinalworkingconcentrationofKMnO4:0.06%
SpecificityDegeneratingneurons,andneuronaldegeneration.Thereisnospecificstaininginnormalhealthybrain.
Cross-reactivityNote:SomeresearchersundersomeconditionsreportbloodvesselstainingwithFluoroJade.ThismaybebecauseFluoroJadeisananalogueofeosin(whichstainsbloodcells).Ingeneral,goodperfusionandpreparationofthetissueshouldhelppreventbloodvesselstainingbutitmaynotbepossibletoeliminateitentirely.Inourexperienceitisgenerallypossibletodistinguishneuronalfrombloodvesselsstainingbyeye.
FormThereagentsintheFluoroJadekitareallsuppliedinaliquidformatandareready-to-dilute.
AppearanceFJCvisualizationisaccomplishedusingbluelightora488nmLaser.ExcitationPeak:495nmEmissionPeak:521nmFiltersystemforvisualizing:Fluorescein/FITC
ReconstitutionDilutesolutionsasdirectedintheprotocolinstructions.Sometimessmallprecipitatesmaybepresentinthestockordilutedsolutions.Completemixingofthedilutedsolutionsusuallydissolvestheprecipitates.Theprecipitates,ifnotremoved,donotusuallycauseanydifficultiesifthewashingstepsarefollowedasinstructed.Optional:ForentirelycleansolutionsBiosensisrecommendsfilteringthedilutedsolutionthoughethanolandNaOHcompatiblesyringeorvacuumfilterdevicespriortocontactwithtissueslides.
StorageTheunopenedkitcanbestoredforupto6monthsat2-8ºCafterthedateofreceipt.Thekitandcomponentsshouldbestoredprotectedfromlight.DilutedFJCdyesolutionsarenotstableandshouldbeusedwithin4hoursofmaking.Theotherdilutedsolutionscanbereusedandstoredforupto48hoursifrefrigeratedandprotectedfromlight.Bestresultsrequirefreshlydilutedsolutions.Werecommendusingaseptictechniqueswhenhandlingthereagentstoavoidbacterialgrowthandcontamination.

TheFJCReadytodilutekitisshippedambientandstableatroomtemperatureduringtransport.Refrigerateuponarrival,donotfreeze.
ExpiryDateUnopenedkit6monthsat2-8ºCprotectedfromlight.SeeStorageinstructionsforworkingsolutionsrecommendations.
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ReagentKitprotocolClick to download the protocolTR-100-FJ_January2017_protocol.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-100-FJ_as_at_May2017.pdf