TheLEGENDplex™HUImmuneCheckpointPanel1(12-plex)isabead-basedmultiplexassaypanel,usingfluorescence-encodedbeadssuitableforuseonvariousflowcytometers.Itallowsforsimultaneousquantificationof12keyimmunecheckpointproteins:sCD25(IL-2Ra),4-1BB,sCD27,B7.2(CD86),FreeActiveTGF-β1,CTLA-4,PD-L1,PD-L2,PD-1,Tim-3,LAG-3,andGalectin-9.ThisassaypanelprovideshigherdetectionsensitivityandbroaderdynamicrangethantrADItionalELISAmethods.Thepanelhasbeenvalidatedforuseoncellculturesupernatant,serum,andplasmasamples.TheLEGENDplex™HUImmuneCheckpointPanel1isconfiguredasshownbelowdependingonsampletypesandrequireddilutions:
CatalogNo. | PlexSize | Targets | Recommended SampleType | Recommended DilutionFactor |
740866 740867 | 12-plex | sCD25(IL-2Ra),4-1BB,sCD27,B7.2(CD86),FreeActiveTGF-β1,CTLA-4,PD-L1,PD-L2,PD-1,Tim-3,LAG-3,Galectin-9 | TissueCulture | Varies |
740961 740962 | 10-plex | sCD25(IL-2Ra),4-1BB,B7.2(CD86),FreeActiveTGF-β1,CTLA-4,PD-L1,PD-1,Tim-3,LAG-3,Galectin-9 | Serum,Plasma | 2 |
740963 740964 | 2-plex | sCD27,PD-L2 | Serum,Plasma | 100 |
TheLEGENDplex™HUImmuneCheckpointPanel1isdesignedtoallowflexIBLecustomizationwithinthepanel.Pleasevisitwww.Biolegend.com/legendplexformoreinformationonhowtomixandmatchwithinthepanel.Thisassayisforresearchuseonly.
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