True-Phos™ Perm Buffer has been developed to permeabilize fixed peripheral blood mononuclear cells (PBMCs), lysed whole blood, cell lines, or splenocytes. Cells can be stimulated cytokines (e.g. IL-4, IL-6, etc.), antibodies (e.g. anti-CD3, clone HIT3a), or phorbol-12-myristate 13-acetate and ionomycin (Cell Activation Cocktail; Cat. No. 423301), and fixed with Fixation Buffer (Cat. No. 420801), or for whole blood, RBC Lysis/Fixation Solution (Cat. No. 422401). This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.). Not all cell surface markers are compatible with BioLegend’s True-Phos™ Perm Buffer.
Product Details Biolegend揭示出对神经系统以及影响神经系统的疾病的更好理解的研究可以转化为改善数百万生命的治疗方法。BioLegend提供广泛的产品组合,为您的神经科学实验提供解决方案,包括抗体,免疫测定溶液,重组蛋白,磁性细胞分离和单细胞蛋白质组学试剂。我们的试剂是与该领域的杰出领导者(如迈克尔·J·福克斯基金会)合作建造的(请参见下面的“产品亮点”标签),这表明我们承诺与您的团队合作寻找新发现,为我们的客户带来新的选择和全面的解决方案研究神经系统疾病的解决方案。