DESCRIPTION:
5Flu-GFCPDHKAAMVLFLDSVYGIEVQDFLLHLLEVGFLP
NegativecontrolforfluoresceinDPc10peptide.
DPc10inactivepeptideisa36-residuepeptidethatdiffersfromDPc10(Gly2460-Pro2495,rabbit) atoneposition(R2475S)andrepresentsthesequenceofaknownCPVTmutantRYR2domain.ThispeptidebindsweaklyorfailstobindtotheDPc10interfaceanddoesNOTaffectchannelfunction.
DPc10inactiveinthecurrentformisnotcellpermeable.TheproductisusefulinapplicationsthatpermitaccesstoRYR2directly(viawholecellpatch,skinnedcells,blacklipidmembrane,vesicles,orupondeliverytocytoplasm).
ReadDPc10Publications
DATASHEET:
Peptidename | FluoresceinDPc10InactivePeptide |
Peptidesequence | 5Flu-GFCPDHKAAMVLFLDSVYGIEVQDFLLHLLEVGFLP |
N-terminus | 5-Carboxyfluorescein |
C-terminus | Acid |
Counterions | Trifluroacetate(TFA) |
Molecularweight | 4093.046 |
HPLCanalysis | Analysiscarriedoutusinga100Å4.6x150mmcolumn,grADIentfrom10%-90%acetonitrile,in15minutes.60degC.Puritydeterminedas97% |
MSanalysis | Molecularionpredicted:4393.046.Observed:4392.6[M+3H] |
Appearance | Yellowpowderedsolid |
Solubilisation | DMSO |
Storage | Storeassuppliedat-20°Cforupto1year.Storedesiccated.Makefreshsolutionspriortouse. |
Datasheet | A010-517ReadDPc10publications |
MSDS | A010-517 |
Notes | Theproductwaspurifiedinacetonitrileandwatercontaining0.1%trifluoroaceticacid(TFA)priortolyophilisation.Theproductwillthereforebepresentasitstrifluoroaceticacidsalt. |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |