DESCRIPTION:
Afullyvalidated,highlyspecificrabbitpolyclonalanti-serumtoSerine-197phosphorylatedformsCalcineurin
CytoplasmicCa2+bindstocalmodulinandactivatesthephosphatasecalcineurin(Cn)a60kDaprotein,andCaMkinaseII(CaMKII).OneactionofCnistodephosphorylatecytoplasmicNFAT(nuclearfactorofactivatedTcells),inducingitstranslocationtothenucleuswhereitactivatesantiapoptoticandhypertrophictargetgenes.ThephosphorylationofCnatPhosphoSer-197sitehasbeenobservedinculturedneonatalratventricularmyocytes(NRVMs)over-expressingconstitutivelyactiveCAMKII.ThisappearstoinhibitCnactivitywhichinturnpreventsthedephosphorylationofNFATandpreventsactivationofNFATregulatedgenes.
Testedapplications:Westernblot(1:200dilution).Speciesrecognition:TheantibodyrecognisesCalcineurininmouse/rat/human/monkey/other,whenphosphorylatedonSer-197.
DATASHEET:
Description | Lyophilisedrabbitpolyclonalanti-serumcontainingIgGantibodyspecificforSer-197phosphorylatedformsCalcineurin |
Immunogen | SyntheticpeptideCGKMARVFS(PO3H2)VLREES-amidecorrespondingtoresiduessurroundingSeratposition197ofCalcineurin |
Isotype | IgG |
Purification | Rawserum |
Speciesreactivity | TheantibodyrecognisesCalcineurininallmammalianspecies,whenphosphorylatedonSer-197.EptiopesequencealsoalignswithchickenandXenopus |
Testedapplications | WB |
Recommendeddilution | WB:1:200 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin100µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-80 |
MSDS | A010-80 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |