DESCRIPTION:
Afullyvalidated,highlyspecificrabbitpolyclonalantibodytoSerine-2814phosphorylatedRyanodineReceptor2
TheRyanodineReceptor(RyR2)isaCa2+channelofcardiacmusclethatplaysacentralroleinECcoupling.ThebindingofCa2+toRyanodineReceptor2RyR2opensthechannelandCa2+storedintheSRmovesthroughthechannelintothecytosoltoinitiatemusclecontraction.CaMKII,wasabletophosphorylateSer-2814ofRYR2enhancingCa2+-sensitivityandincreasingopenprobABIlity.ExcessivephosphorylationofSer-2814leadstoatrialfibrillation,arrhythmiasandsuddendeath
Testedapplications: Westernblot(1:5000dilution),immunofluorescencemicroscopy(1:100). Speciesrecognition: Allmammalianspecies,whenphosphorylatedonSer-2814(orSer-2815rabbit)Antibodyrecognisesepitopedespitesingleresiduedifferenceinepitopebetweenspecies(V2815I;humanvsmouse).
DATASHEET:
Description | Lyophilisedrabbitanti-serum |
Immunogen | Syntheticpeptide(TSQVS(PO3H2)VDAAH2819)correspondingtoaminoacidssurroundingthephosphorylatedserineresidueatposition2814ofRyR2(human)conjugatedtoKLH. |
Isotype | IgG |
Purification | Rawserum |
Speciesreactivity | PeptidealignswithRyR2fromallmammalianspecies.Sequencecorrespondsexactlyinrabbitbutdiffersatoneresidue(V2815I)inmouseandrat.Howeverantibodyrecognisesbothsequences. |
Testedapplications | WB,IHC |
Recommendeddilution | WB1:5000,IHC1:100 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°CReconstituted:-80°C |
Datasheet | A010-31Lot1209-04 A010-31Lot0513-06 |
MSDS | A010-31 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |