DESCRIPTION:
Afullyvalidated,highlyspecificaffinitypurifiedmousemonoclonalantibodytoskeletalmuscleSERCA1
Background:SERCA1istheCa2+-pump(109kDa)locatedinthesarcoplasmicreticulum(SR)offast-twitchskeletalmuscle.Theenzymetransports2Ca2+fromthecytoplasmintotheSRusingthehydrolysisof1moleculeofATPtopowerthereaction.Thisterminatesmusclecontractionandpromotesmusclerelaxation.MutationsintheSERCA1gene(ATP2A1)areobservedinsomepatientswithBrodydisease,anexerciseinducedimpairmentinmusclerelaxtion,stiffnessandcramps.ThemonoclonalantibodyB/4H3wasproducedbyimmunizingmicewithpurifiedSERCA1:theantibodyinhibitsSERCA1activityuponbinding
TestedApplications:WesternBlot(1:5000dilution),IHCMicroscopy(1:200dilution).Speciesrecognition:AntibodytestedonRatandrabbittissue;immunogenicpeptidealignswithMammalianspecies,Avian,AmphibianandFish
DATASHEET:
Description | AffinitypurifiedMousemonoclonalantibodytoSERCA1 |
Immunogen | PurifiedSERCA1a.Epitopewasmappedtoresidues662-666 |
Isotype | IgG |
Purification | ProteinAaffinitypurified |
Speciesreactivity | AntibodytestedonRatandRabbittissue,butimmunogenicpeptidealignswithMammalianspecies,Avian,AmphibianandFish |
Testedapplications | WB |
Recommendeddilution | WB1:5000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin50µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-22AP |
MSDS | A010-22AP |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |