DESCRIPTION:
Highlyspecific,wellvalidatedpolyclonalantiserumtoSERCA2a
SERCA2aisaCa2+pumplocatedinthesarcoplasmicreticulumofcardiacandslow-twitchskeletalmuscle.TheenzymetransportsCa2+fromthecytoplasmintotheSRusingthehydrolysisofATPastheenergysourceforthisreaction.TwoCa2+aretransportedforeachATPhydrolysed. ThisCa2+transportistheprincipalmeansofmusclerelaxationincardiacandslow-twitchskeletalmuscle.TheactivityofSERCA2aismoderatedbyitsinteractionwithphospholamban.SERCA2aisoneisoformofSERCA2:thisisoformdiffersfromSERCA2bandSERCA2cattheextremecarboxyterminusonly. AntibodyA010-20recognisestheC-terminalsequencewhichdistinguishesSERCA2afromSERCA2bandSERCA2c.
Testedapplications: WesternBlot(1:5000dilution). Speciesrecognition: TheantibodyreactswithSERCA2afromhuman,rat,mouse,rabbit,canine,andbovinespecies.
DATASHEET:
Description | LyophilisedRabbitpolyclonalserumcontainingIgGantibodyspecifictoSERCA2a |
Immunogen | Syntheticpeptide(C-LEPAILE997)correspondingtoaminoacidsattheextremeC-terminusofSERCA2a |
Isotype | IgG |
Purification | Rawserum |
Speciesreactivity | TheantibodyrecognisesSERCA2aandbindingisblockedinthepresenceofapeptidecontainingtheepitope.TheantibodyreactswithSERCA2afromhuman,rat,mouse,rabbit,canine,andbovinespecies. |
Testedapplications | WB,ELISAandIHCmicroscopy |
Recommendeddilution | WB1:5000 |
Optimization | Optimaldilutionstobedeterminedbyenduser |
Storage | Lyophilisedantibodyisstableat4°Cwhenstoredwithdesiccant.Reconstitutelyophilisedpowderin20µlof18MΩH2O,aliquotandstorefrozenat-80°Cfor1year.Avoidfreeze-thawcycles. |
Storage | Lyophilised:4°C.Reconstituted:-80°C |
Datasheet | A010-20Lot0210-06 A010-20Lot0814-01 |
MSDS | A010-20 |
Regulatorystatement | Productforresearchuseonly.Notintendedfordiagnosticortherapeuticuse. |