AxolFibronectinCoatingSolutioncanbeusedtopromoteattachmentofawidevarietyofcellstypes.IthasbeenvalidatedtopromoteattachmentofAxoliPSC-DerivedVentricularCardiomyocytesafterthawing.
Thehumanfibronectincoatingsolutioniscellculturetestedandisnegativeforhumanvirusesandmicrobialcontamination.
Therecommendedworkingconcentrationvariesdependingoncelltype:
ax2015HumaniPSC-DerivedEndothelialColonyFormingCells1:100
ax2019HumaniPSC-DerivedEndothelialColonyFormingCells1:100
ax2505HumaniPSC-DerivedVentricularCardiomyocytes 1:100 (canbefurtheroptimizedforindividualexperiments)
ax9001MesenchymalStemCells(MSCs)(pre-ADIpocyte)1:50 ax9002MSCs(adiposetissue-derived) 1:50
ax9003MSCs(umbilicalcord-derived)1:50
ax9004MSCs(bonemarrow-derived)1:50
DiluteFibronectinCoatingSolutioninsterilewaterandmixwell.
Completelycoverthewellstocoatandincubateovernightat37oCinahumidifiedincubator.Aspiratethecoatingsolutionpriortoseedingthecells.
Oncediluted,useimmediatelyasdilutedFibronectinCoatingSolutionisnotstableforextendedperiodsoftime.
FibronectinCoatingSolutionshouldbestoredinsmallconcentratedaliquotstoreducefreeze-thawcycles.