Description:
NeuralStemCellsfromaTrisomyXPatient
TrisomyX(TripleX)syndromeoccursatafrequencyofapproximately1in1000newbornfemales.Thesyndromeisassociatedwithanincreasedriskofseizuresandmayaffectshort-termmemoryandlearningalthoughthemajorityofwomenaffectedshowfewclinicalsymptoms.AxolhasdevelopediPSC-derivedneuralstemcellsfromapatientwiththetripleXchromosomalabnormality.AlloftheiPScellsexamined(20individualcellskaryotyped),hadtrisomyX.
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.
ProductSpecification:
Startingmaterial | Dermalfibroblast |
Donorgender | Female |
Donorageatsampling | 74yrs |
Karyotype | TrisomyX(47,XXX) |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | None |
Size | ≥1.5millioncells |
Celltype | iPSC-derivedneuralstemcells |
Growthproperties | Adherent |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |