LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:m,r
Mayalsoworkin:h
ImmunogenPeptide(C)DAGNSSWNGTEAPG,correspondingtoaminoacidresidues7-20ofratα2A-adrenoceptor(AccessionP22909).Extracellular,N-terminus.
Accession(Uniprot)NumberP22909
GeneID25083
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-identical;human-13/14aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.85mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ih,wb
Mayalsoworkin:ic,ifc,ip,lci
WesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:
1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofα2A-Adrenoreceptorinratcerebellum
ImmunohistochemicalstainingofratcerebellumfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020).A.α2A-Adrenoreceptorstaining(green)appearsinprocessesofBergmannglia(whitearrows)inthemolecularlayer(ML).B.Stainingofthesamesectionwithmouseantiglialfibrillaryacidicprotein(GFAP)(red),demonstratesexpressioninbothBergmanngliaandingranulelayer(GL)astrocytes.C.MergedimageofpanelAandBdemonstratingthatα2A-adrenoreceptorexpressionisrestrictedtoBergmannglia.DAPIisusedasthecounterstain(blue).
Expressionofα2A-Adrenoreceptorinrathypothalamus
ImmunohistochemicalstainingofratventromedialhypothalamusfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:250).A.α2A-Adrenoreceptorstaining(green)appearsinastrocyticfibers(arrows)originatingfromthewallofthe3rdventricle(3rdV).B.DAPIisusedasthecounterstain(blue).C.MergedimageofpanelsAandB.
ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS
Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.
CivantosCalzada,B.andAleixandredeArtinano,A.(2001)Pharmacol.Res.44,195.
Bylund,D.B.(2005)Br.J.Pharmacol.144,159.
ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.
AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.
Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.
Ingeneral,thepharmacologicalpropertiesofeachsubtypearequitehomogenousacrossdifferentspecies.However,thisisnotthecasefortheα2A subtypewhichwasfirstisolatedfromhumanplatelets3.Thissubtypeshowsdifferentpharmacologicalpropertiesfromthatofmouseandrat.Forthisreason,untilmoleculartechniquessignificantlyadvanced,itwasbelievedthathumanα2A andthatofmouseandrat(termedα2D)weretwodifferentsubtypes.Today,itisacceptedthatthesetwosubtypesareinfactonegeneproductandisgenerallytermedα2A.Oneofthemaindifferencesbetweenthissubtypefromthedifferentorganismsisitsaffinityforyohimbineandrauwolscine;ratandmouseα2A displayslessaffinitycomparedtohuman3,4.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:04/11/2018
Anti-α2A-AdrenergicReceptor(extracellular)Antibody(#AAR-020)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2A-adrenoceptorfrommouse,rat,andhumansamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:
1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).
3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
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Alomone英文简介：
Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
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