LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
ShippedatRoomTemp.ShippedatRoomTemp.
Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.
Type:Polyclonal
Source:Rabbit
Reactivity:h,r
Mayalsoworkin:m
ImmunogenPeptide(C)KETGAFYGREFKTAK,correspondingtoaminoacidresidues216-230ofhumanA3AR(AccessionP0DMS8).3rdintracellularloop.
Accession(Uniprot)NumberP0DMS8
GeneID140
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyRat,mouse -14/16aminoacidresiduesidentical.
PurityAffinitypurifiedonimmobilizedantigen.
FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.7mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Controlantigenreconstitution100µldoubledistilledwater(DDW).
Controlantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,ih,wb
Mayalsoworkin:ifc,ip
WesternblotWesternblotanalysisofMalme-3Mcelllysate:
1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
ImmunohistochemistryExpressionofAdenosine A3 Receptorinratlung
Immunohistochemicalstainingofparaffin-embedded ratlungsectionsusingAnti-Adenosine A3 Receptor Antibody(#AAR-004),(1:50).Stainingisspecificforrespiratoryepithelialcellsofthebronchiole(arrows)andpneumonocytesinthealveolarwall(a).Hematoxilinisusedasthecounterstain.
ImmunocytochemistryExpressionofAdenosine A3 Receptorinahumanmelanomacellline
Immunocytochemicalstainingofparaformaldehyde-fixedandperme
ABIlized humanmelanomacells(A2058).A,D.Cellswerestainedusing
Anti-Adenosine A3 Receptor Antibody(#AAR-004),(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B,E.Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342.C.MergedimageofpanelsAandB.F.MergedimageofpanelsDandE.
ReferencesOkusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.
Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.
Nakata,H.(1989)J.Biol.Chem.264,16545
Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.
Headrick,J.P.etal.(2003)Am.J.Physiol.HeartCirc.Physiol.285,H1797.
M
ADI,L.
etal.(2004)
Clin.CancerRes.10,4472.
Ochaion,A.etal.(2007)Ann.Rheum.Dis.66,446.
ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor (A1AR), AdenosineA2A Receptor (A2AAR),AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.1-4 AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.1-3
ExpressionofA3ARhasbeenreportedinthebrain,kidney,liver,andheart.Itplaysaroleinmodulationofcerebralischemia,asthma,andcellgrowth.Inaddition,recentstudieshaveestablishedacardioprotectiveroleforA3AR.5 ExpressionofA3ARwasreportedtobeelevatedincanceroustissuesaswellasinauto-immuneinflammatorydiseases.6,7 ApatenthasbeenfiledfortheuseofA3ARandA3ARagoNISTasadiagnosticMarkerfortherapeutictreatmentsofcancerandotherdiseases.
LyophilizedPowderControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:26/02/2019
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanintracellularepitopeofthehumanadenosineA3 receptor(A3AR).Anti-Adenosine A3 ReceptorAntibody(#AAR-004)canbeusedinwesternblotanalysis,aswellasimmunocytochemicalandimmunohistocemicalapplications,andhasbeendesignedtorecognizeA3ARfromhuman,rat,andmousesamples.
Forresearchpurposesonly,notforhumanuse
WesternblotWesternblotanalysisofMalme-3Mcelllysate:
1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).
2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.
Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列**的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的*重要的抗体和生长因子）。
Alomone成立于1989年,已经成为全世界科学家信赖的***的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。
Alomone公司是一家位于耶路撒冷的以色列杰出的生物科技公司，专著于提供生命科学细胞生物学试剂和服务。
Alomone公司作为世界上*杰出的离子通道专业供应商，它的离子通道抗体是世界上*全的、质量也非常好！目前被世界各地的科学家广泛的使用。
Alomone英文简介：
Alomone Labs, your partner in ion channel research, is a leading supplier of Ion Channel, GPCR and Neural Signaling tools. We specialize in the production of primary antibodies, small molecules, proteins and peptides used in basic science research. Alomone was established in 1989 and has become the most comprehensive source of ion channel research tools used by scientists worldwide. All of Alomone`s products undergo bio***** validation and are supplied in lyophilized powder format so that you can be confident about their biological activity and long shelf life.
Alomone部分产品列表：
Alomone Anti-Zinc-Activated Channel (ZAC) (extracellular)
Alomone Anti-Zinc-Activated Channel (ZAC) (extracellular)
Alomone Anti-Vasopressin V2 Receptor
Alomone Anti-Vasopressin V2 Receptor
Alomone Anti-Vasopressin V2 Receptor
Alomone Anti-Vasopressin V1B Receptor
Alomone Anti-Vasopressin V1B Receptor
Alomone Anti-Vasopressin V1B Receptor
Alomone Anti-Vasopressin V1A Receptor
Alomone Anti-Vasopressin V1A Receptor
Alomone Anti-Vasopressin V1A Receptor
Alomone Anti-PAC1 Receptor
Alomone Anti-PAC1 Receptor
Alomone Anti-PAC1 Receptor
Alomone Anti-VPAC2 (extracellular)
Alomone Anti-VPAC2 (extracellular)
Alomone Anti-VPAC1 (extracellular)
Alomone
Anti-VPAC2 (extracellular)
Alomone
Anti-VPAC1 (extracellular)
Alomone
Anti-VPAC1 (extracellular)
Alomone
Anti-Pan Voltage Dependent Anion Channels Antibody
Alomone
Anti-Trace Amine Receptor 1
Alomone
Anti-Pan Voltage Dependent Anion Channels Antibody
Alomone
Anti-Trace Amine Receptor 1
Alomone
Anti-Trace Amine Receptor 1
Alomone
Anti-TSH Receptor (extracellular)
Alomone
Anti-TSH Receptor (extracellular)
Alomone
Anti-TSH Receptor (extracellular)
Alomone
Anti-Thyrotropin-Releasing Hormone Receptor 1
Alomone
Anti-Thyrotropin-Releasing Hormone Receptor 1
Alomone
Anti-Neurokinin Receptor 2 (NK2)
Alomone
Anti-Neurokinin Receptor 2 (NK2)
Alomone
Anti-Neurokinin Receptor 2 (NK2)
Alomone
Anti-Neurokinin Receptor 1 (NK1) (extracellular)-ATTO-488
Alomone
Anti-Neurokinin Receptor 1 (NK1) (extracellular)
Alomone
Anti-Neurokinin Receptor 1 (NK1) (extracellular)
Alomone
Anti-TMC6 (EVER1)
Alomone
Anti-TMC6 (EVER1)
Alomone
Anti-TMC6 (EVER1)
Alomone
Anti-TRIC-A
Alomone
Anti-TRIC-A
Alomone
Anti-TRIC-B
Alomone
Anti-TRIC-B
Alomone Anti-5-Hydroxytryptamine Receptor 4 (extracellular)
Alomone Anti-5-Hydroxytryptamine Receptor 4 (extracellular)
Alomone Anti-5-Hydroxytryptamine Receptor 4 (extracellular)
Alomone Anti-5-Hydroxytryptamine Receptor 2B (extracellular)
Alomone Anti-5-Hydroxytryptamine Receptor 2B (extracellular)
